Summary
The responses of two potato cultivars, Crystal and Norgold Russet, to a standard protoplast regeneration procedure were examined. Both cultivars yielded protoplasts that survived and grew in the initial plating medium. Protoplast-derived callus tissue also grew on the callus proliferation medium. The cultivars differed most in their ability to survive on the shoot induction medium; changes in its growth regulator content and the quantity of agar increased shoot production of Crystal from 1% to 30% and that of Norgold Russet from 7% to 24% on modified medium under low light intensity.
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Journal Series Paper 1699 of the North Dakota Agricultural Experiment Station.
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Taylor, R.J., Secor, G.A. A shoot induction procedure altered for increased shooting efficiency of potato protoplast-derived calli (cvs. crystal and norgold russet). Potato Res 31, 651–658 (1988). https://doi.org/10.1007/BF02361858
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DOI: https://doi.org/10.1007/BF02361858