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Potato Research

, Volume 39, Issue 2, pp 271–282 | Cite as

General resistance against potato virus Y introduced into a commercial potato cultivar by genetic transformation with PVYN coat protein gene

  • Daisaku Okamoto
  • Søren V. S. Nielsen
  • Merete Albrechtsen
  • Bernhard Borkhardt
Article

Summary

Transgenic cv. Folva potato plants expressing the coat protein gene of potato virus Y strain N (PVYN) were produced usingAgrobacterium tumefaciens mediated transformation. Forty independent transformants were selected for resistance screening. Four clones showed complete resistance to mechanical inoculation with all the five PVY isolates tested: the PVYN isolate from which the coat protein gene was derived, two PVYO isolates, and two PVYNTN isolates. Two of the fully resistant clones contained only one copy of the transgene, demonstrating that it is possible by genetic engineering to obtain highly virus resistant potato clones that can also be useful in future breeding programmes.

Additional keywords

Pathogen derived resistance genetic engineering Solanum tuberosum

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Daisaku Okamoto
    • 1
  • Søren V. S. Nielsen
    • 1
  • Merete Albrechtsen
    • 1
  • Bernhard Borkhardt
    • 1
  1. 1.The Biotechnology GroupDanish Institute of Plant and Soil ScienceLyngbyDenmark

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