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General resistance against potato virus Y introduced into a commercial potato cultivar by genetic transformation with PVYN coat protein gene

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Summary

Transgenic cv. Folva potato plants expressing the coat protein gene of potato virus Y strain N (PVYN) were produced usingAgrobacterium tumefaciens mediated transformation. Forty independent transformants were selected for resistance screening. Four clones showed complete resistance to mechanical inoculation with all the five PVY isolates tested: the PVYN isolate from which the coat protein gene was derived, two PVYO isolates, and two PVYNTN isolates. Two of the fully resistant clones contained only one copy of the transgene, demonstrating that it is possible by genetic engineering to obtain highly virus resistant potato clones that can also be useful in future breeding programmes.

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Author notes

  1. Daisaku Okamoto

    Present address: Takii Naganuma Breeding Station, Kita 17, Higashi 4, Naganuma Yubari, 069-13, Hokkaido, Japan

Authors and Affiliations

  1. The Biotechnology Group, Danish Institute of Plant and Soil Science, Lottenborgvej 2, DK-2800, Lyngby, Denmark

    Daisaku Okamoto, Søren V. S. Nielsen, Merete Albrechtsen & Bernhard Borkhardt

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  1. Daisaku Okamoto
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  2. Søren V. S. Nielsen
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  3. Merete Albrechtsen
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  4. Bernhard Borkhardt
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Okamoto, D., Nielsen, S.V.S., Albrechtsen, M. et al. General resistance against potato virus Y introduced into a commercial potato cultivar by genetic transformation with PVYN coat protein gene. Potato Res 39, 271–282 (1996). https://doi.org/10.1007/BF02360919

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