Summary
The changes in polypeptide profiles (2D-PAGE) occurring in the soluble and microsomal fractions of parenchymatic tissue of potato (Solanum tuberosum L.) tubers were studied during the last 30 d of maturation and during storage at 23°C and 3°C. The major changes were observed in the last period of tuber maturation, when several polypeptides disappeared and new ones appeared. At both 23°C and 3°C specific polypeptides disappeared in dormant tubers and new polypeptides appeared during storage. At 3°C specific changes in protein composition occurred, particularly in the microsomal fraction. The changes in polypeptide profiles are discussed in relation to the transition from “sink” to “source” of the tuber, the onset of dormancy and of sprouting ability and the activation of cold acclimation responses. The results are also discussed on the basis of the physiological and biochemical changes that occur in the parenchymatic tissue.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berkel, J. van, F. Salamini & C. Gebhardt. 1994. Transcripts accumulating during cold storage of potato (Solanum tuberosum L.) tubers are sequence related to stress-responsive genes.Plant Physiology 104: 445–452.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry 72: 248–254.
Borgmann, K., P. Sinha & W.B. Frommer, 1994. Changes in two-dimensional protein pattern and in gene expression during the sink-to-source transition of potato tubers.Plant Science 99: 97–108.
Cocucci, S.M., F.M. Garlaschi., E. Bianchi & E. Marrè, 1972. Changes of the protein synthesis system during the maturation of potato tuber.Physiologia Plantarum 27: 220–225.
Davies, H.V., 1990. Carbohydrate metabolism during sprouting.American Potato Journal 67: 815–827.
Davies, H.V. & H.A. Ross, 1984. The pattern of starch and protein degradation in tubers.Potato Research 27: 373–381.
Désiré, S., J.P. Couillerot, J.L. Hilbert & J. Vasseur, 1995. Protein changes inSolanum tuberosum during storage and dormancy breaking ofin vitro microtubers.Plant Physiology and Biochemistry 33: 479–487.
Dyer, W.E., 1993. Dormancy-associated embryonic mRNAs and proteins in imbibingAvena fatua caryopses.Physiologia Plantarum 88: 201–211.
Es, A. van & K.J. Hartmans, 1987. Starch and sugars during tuberization, storage and sprouting. In: A. Rastovski, A. van Es et al. (Eds) Storage of Potatoes. Post-harvest Behaviour, Store Design, Storage Practice, Handling. Pudoc, Wageningen, pp. 79–113.
Espen, L., S. Morgutti, A. Abruzzese, N. Negrini, A. Rivetta, M. Quattrini, M. Cocucci & S.M. Cocucci, 1999. Changes in the potato (Solanum tuberosum L.) tuber at the onset of dormancy and during storage at 23°C and 3°C: I. Biochemical and physiological parameters.Potato Research 42: 189–201.
Guy, C.L., 1990. Cold acclimation and freezing stress tolerance: role of protein metabolism.Annual Review of Plant Physiology and Plant Molecular Biology 41: 187–223.
Hance, B.A. & J.M. Bevington, 1992. Changes in protein synthesis during stratification and dormancy release in embryos of sugar maple (Acer saccharum).Physiologia Plantarum 86: 365–371.
Heukeshoven, J. & R. Dernick, 1988. Improved silver staining procedure for fast staining in PhastSystem development unit.Electrophoresis 9: 28–32.
Hughes, M.A. & M.A. Dunn, 1996. The molecular biology of plant acclimation to low temperature.Journal of Experimental Botany 47: 291–305.
Knowles, L.O. & N.R. Knowles, 1990. Changes in fatty acid composition of phospholipids from different ages of potato seed-tubers during sprouting.Annals of Botany 65: 217–223.
Li, B. & M.E. Foley, 1997. Genetic and molecular control of seed dormancy.Trends in Plant Science 2: 384–389.
Mayer, J.E., G. Hahne, K. Palme & J. Schell, 1987. A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis.Plant Cell Reports 6: 77–81.
Plas, L.H.W. van der, 1987. Potato tuber storage: biochemical and physiological changes. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry. Vol. 3: Potato. Springer-Verlag, Berlin Heidelberg, pp. 109–135.
Reust, W., 1986. EAPR. Working group: physiological age of the potato.Potato Research 29: 268–271.
Schneider, A., F. Salamini & C. Gebhardt, 1997. Expression patterns and promoter activity of the cold-regulated geneci21A of potato.Plant Physiology 113: 335–345.
Schneider, W.L. & D.J. Gifford, 1994. Loblolly pine seed dormancy. I. The relationship between protein synthesis and the loss of dormancy.Physiologia Plantarum 90: 246–252.
Yoshida, S. & M. Uemura, 1990. Responses of the plasma membrane to cold acclimation and freezing stress. In: C. Larsson & I.M. Möller (Eds), The Plant Plasma Membrane. Springer-Verlag. Berlin Heidelberg, pp. 293–319.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Espen, L., Morgutti, S. & Cocucci, S.M. Changes in the potato (Solanum tuberosum L.) tuber at the onset of dormancy and during storage at 23°C and 3°C. II. Evaluation of protein patterns. Potato Res 42, 203–214 (1999). https://doi.org/10.1007/BF02358410
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02358410