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A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis

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Abstract

A fast and simple extraction procedure of plant tissue for two-dimensional gel electrophoresis is presented. The procedure is especially useful for the extraction of plant cell suspension cultures, callus and other plant tissues having a high content of phenol oxidases, polysaccharides, polynucleotides, terpenoids and other substances interfering with isoelectric focusing. Due to the speed of the extraction procedure (about 20 min), large numbers of samples containing only milligram amounts of tissue can be easily processed. The simplicity of the method makes it particularly suitable for the extraction of radiolabeled tissues (35S, 32P). This method is perfectly compatible with silver staining, autoradiography and Western blotting analysis.

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Abbreviations

DTT:

dithiothreitol

IEF:

isoelectric focusing

SDS:

sodium dodecyl sulfate

TEMED:

N,N,N′,N′-tetramethylethylenediamine

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Authors and Affiliations

  1. Max-Planck Institut für Züchtungsforschung, D-5000, Köln 30, Federal Republic of Germany

    Jorge E. Mayer, Günther Hahne, Klaus Palme & Jeff Schell

Authors
  1. Jorge E. Mayer
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  2. Günther Hahne
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  3. Klaus Palme
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  4. Jeff Schell
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Additional information

Communicated by W. Barz

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Mayer, J.E., Hahne, G., Palme, K. et al. A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis. Plant Cell Reports 6, 77–81 (1987). https://doi.org/10.1007/BF00269745

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  • DOI: https://doi.org/10.1007/BF00269745

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