Summary
Using visual selection for off-types and image analysis to select against maturation mutants, a line combining improved late blight resistance and yield was isolated in an in vitro mutagenesis programme from a population of 2101 putative mutants. The adventitious regenerants from mutagenesis treatment, including spontaneous mutants (somaclonal variants), were subjected to serial subculture of the apical bud in vitro and two cycles of apical cuttings in vivo to breakdown chimeras. The selected line was stable in trials over five years; others were unstable or failed to combine improvement in late blight resistance with high yield. Leaf and tuber image analysis was used to characterize selected lines. Lines that differed most from cv. Golden Wonder in yield and late blight resistance were more easily distinguished from the control by image analysis. The role of image analysis in negative selection and in determination of the relatedness of mutants to the control is discussed.
Similar content being viewed by others
References
Bradshaw, J.E. & G.R. Mackay, 1994. Potato genetics. CAB International, Wallingford.
Cassells, A.C., 1998.In-vitro induced mutations for disease resistance. In: S. Mohan Jain, B.S. Brar & B.S. Ahloowalia (Eds), Somaclonal Variation and Induced Mutation in Crop Improvement. Kluwer, Dordrecht, pp. 367–378.
Cassells, A.C. & P.T. Sen, 1996.In vitro production of late blight (Phytophthora infestans)-resistant potato plants. In: Y.P.S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry 36. Springer-Verlag, Berlin, pp. 107–118.
Cassells, A.C., S. Austin & E.M. Goetz, 1987. Variation in tubers in single cell-derived clones of potato in Ireland. In: Y.P.S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry 3 Potato. Springer-Verlag, Berlin, pp. 375–391.
Cassells, A.C., C. Walsh & C. Periappuram, 1993. Diplontic selection as a positive factor in determining the fitness of mutants ofDianthus ‘mystere’ derived from X-irradiation of nodes inin vitro culture.Euphytica 70:167–174.
Cassells, A.C., M.L. Deadman, C.A. Brown & E. Griffin, 1991. Field resistance to late blight (Phytophthora infestans (Mont.) De Bary) in potato (Solanum tuberosum L.) somaclones associated with instability and pleiotropic effects.Euphytica. 56:75–80.
Curry, R.F. & A.C. Cassells, 1999. Callus initiation, maintenance and shoot induction in potato: monitoring of spontaneous genetic variabilityin vitro andin vivo. In: R.H. Hall (Ed.), Methods in Molecular Biology: Plant Cell Culture Protocols. Humana Press, New York, pp. 31–42.
Dowley, L.J. & E. O'Sullivan, 1995. Potato Late Blight Control. Agriculture and Food Development Authority, Dublin.
Frost, C. (Ed.), 1984. Maincrop Potato Production. The Agricultural Institute, Dublin.
Fry, W.E., 1978. Quantification of general resistance of potato cultivars and fungicide effects for integrated control of potato late blight.Phtytopathology, 68:1650–1655.
Howard, H.W., 1978. The production of new varieties. In: P.M. Harris (Ed.), The Potato Crop. Chapman & Hall, London, pp. 606–646.
James, W.C., 1971. A manual of assessment keys for plant diseases. Canadian Department of Agriculture, Ottawa.
Jones, P.W., 1990.In vitro selection of disease resistance. In: P.J. Dix (Ed.) Cell Line Selection. VCH, Weinheim, pp. 113–149.
Kowalski, B. & A.C. Cassells, 1995. Novel sources of resistance to late blight of potato. In: L.J. Dowley, E. Bannon, L.R. Cooke, T. Keane, & E. O'Sullivan (Eds), Phytophthora infestans 150. European Association of Potato Research — Pathology Section Conference, Dublin, Ireland, September 1995. Boole Press Ltd., pp. 303–310.
Wenzel, G. & B. Foroughi-Wehr, 1993.In vitro selection. In: M.D. Hayward, N.O. Bosemark & I. Romagosa (Eds), Plant Breeding. Chapman & Hall, London. pp. 353–370.
Zaag, D.E. van der, 1994. The need for international research programmes on some potato pathogens.Potato Research 37:323–329.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kowalski, B., Cassells, A.C. Mutation breeding for yield andPhytophthora infestans (Mont.) de Bary foliar resistance in potato (Solanum tuberosum L. cv. Golden Wonder) using computerized image analysis in selection. Potato Res 42, 121–130 (1999). https://doi.org/10.1007/BF02358402
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02358402