Summary
X-irradiation of in vitro nodes of Dianthus ‘Mystère’ resulted in the production of useful flower colour variants at a rate of approximately 2%. The breakdown of chimeras under diplontic selection and putative selection for fitness in the mutant homohistonts was facilitated by in vitro subculture. The resultant mutants were vigorous and most were stable although pleiotropy in respect to Alternaria disease was observed.
Image analysis of leaf shape in the mutagenised progeny detected no significant differences in the population means for this character but significant increase in the standard deviation was correlated with increase in dose rate. Significant differences in means for leaf shape were detected between the control and selected flower colour-variant lines and between many of the lines indicating that the flower colour changes were occurring in a background of quantitative gene changes in the variants.
The comparative advantages of this model of in vitro mutagenesis are discussed.
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Cassells, A.C., Walsh, C. & Periappuram, C. Diplontic selection as a positive factor in determining the fitness of mutants of Dianthus ‘Mystère’ derived from x-irradiation of nodes in in vitro culture. Euphytica 70, 167–174 (1993). https://doi.org/10.1007/BF00023756
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DOI: https://doi.org/10.1007/BF00023756