Skip to main content
SpringerLink
Log in

Characterisation of genes isolated from a potato swellingstolon cDNA library

  • Full Papers
  • Published:
Potato Research Aims and scope Submit manuscript

Summary

In screening to isolate a full-length copy of a previously isolated cDNA clone, a further three cDNAs were also isolated from a library prepared from sub-apical swelling-stolon tissue of potato (Solanum tuberosum L.). Sequence analysis showed these clones to be similar to extensin-like protein genes, acyl carrier protein thioesterase genes and high mobility group protein genes, respectively. A further cDNA, isolated by subtractive hybridisation, was similar to a tomato cDNA previously isolated on the basis of its down-regulation following nematode infection. While all the newly isolated genes were expressed in swelling stolons, for most, maximal expression was seen to be in stem tissue. Possible roles for these genes in the development of potato plants are discussed, as is the significance of gene expression in stems and stolons to the process of tuberisation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altschul, S.F., W. Gish, W. Miller, E.W. Myers & D.J. Lipman, 1990. Basic local alignment search tool.Journal of Molecular Biology 215:403–410.

    Article  CAS  PubMed  Google Scholar 

  • Artschwager, E., 1924. Studies on the potato tuber.Journal of Agricultural Research 27: 809–835.

    Google Scholar 

  • Bachem, C.W.B., R.S. van der Hoeven, S.M. de Bruijn, D. Vreugdenhil, M. Zabeau & R.G.F. Visser, 1996. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP, Analysis of gene expression during potato tuber development.Plant Journal 9:745–753.

    Article  CAS  PubMed  Google Scholar 

  • Balamani, V., K. Veluthambi & B.W. Poovaiah, 1986. Effect of calcium on tuberization in potato (Solanum tuberosum L.).Plant Physiology 80:856–858.

    CAS  Google Scholar 

  • Cutter, E.G., 1992. Structure and development of the potato plant. In: P.M. Harris (Ed.), The Potato Crop. Chapman & Hall, London, pp. 65–161.

    Google Scholar 

  • Devereux, J., P. Haeberli & O. Smithies, 1984. A comprehensive set of sequence analysis programs for the VAX.Nucleic Acids Research 12:387–395.

    CAS  PubMed  Google Scholar 

  • Ewing, E.E. & P.C. Struik, 1992. Tuber formation in potato: induction, initiation and growth.Horticultural Reviews 14:89–198.

    Google Scholar 

  • Goldman, M.H., M. Pezzotti, J. Seurinck & C. Mariani, 1992. Developmental expression of tobacco pistil-specific genes encoding novel extensin-like proteins.Plant Cell 4:1041–1051.

    CAS  PubMed  Google Scholar 

  • Grasser, K.D., 1995. Plant chromosomal high mobility group (HMG) proteins.Plant Journal 7:185–192.

    Article  CAS  PubMed  Google Scholar 

  • Gregory, L.E., 1956. Some factors for tuberization in the potato plant.American Journal of Botany 43:281–288.

    CAS  Google Scholar 

  • Höfgen, R. & L. Willmitzer, 1992. Transgenic potato plants depleted for the major tuber protein patatin via expression of antisense RNA.Plant Science 87:45–54.

    Google Scholar 

  • Jackson, S.D., A. Heyer, J. Dietze & S. Prat, 1996. Phytochrome B mediates the photoperiodic control of tuber formation in potato.Plant Journal 9:156–166.

    Article  Google Scholar 

  • Jarvis, S.B., M.A. Taylor, M.R. MacLeod & H.V. Davies, 1996. Cloning and characterisation of the cDNA clones of three genes that are differentially expressed during dormancy-breakage in the seeds of Douglas fir (Pseudotsuga menziesii).Journal of Plant Physiology 147:559–566.

    CAS  Google Scholar 

  • Kang, S.-G. & D.J. Hannapel, 1996. A novel MADS-box gene of the potato (Solanum tuberosum L.) expressed during the early stages of tuberization.Plant Molecular Biology 31:379–386.

    Article  CAS  PubMed  Google Scholar 

  • Koda, Y., Y. Kikuta, H. Tazaki, Y. Tsujino, S. Sakamura & T. Yoshihara, 1991. Potato tuber-inducing activities of jasmonic acid and related compounds.Phytochemistry 30:1435–1438.

    Article  CAS  Google Scholar 

  • Kumar, A., M.A. Taylor, S.A. Mad Arif & H.V. Davies, 1996. Potato plants expressing antisense and sense S-adenosylmethionine decarboxylase (SAMDC) transgenes show altered levels of polyamines and ethylene: antisense plants display abnormal phenotypes.Plant Journal 9:147–158.

    Article  CAS  Google Scholar 

  • Lovell, P.H. & A. Booth, 1967. Effects of gibberellic acid on growth, tuber formation and carbohydrate distribution inSolanum tuberosum.New Phytologist 68:1175–1185.

    Google Scholar 

  • MacIntosh, G.C., R.M. Ulloa, M. Raíces & M.T. Téllez-Iñón, 1996. Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato.Plant Physiology 112:1541–1550.

    CAS  PubMed  Google Scholar 

  • Mad Arif, S.A., M.A. Taylor, L.A. George, A.R. Butler, L.R. Burch, H.V. Davies, M.J.R. Stark & A. Kumar, 1994. Characterisation of the S-adenosylmethionine decarboxylase (SAMDC) gene of potato.Plant Molecular Biology 26:327–338.

    Article  CAS  PubMed  Google Scholar 

  • Müller-Röber, B., U. Sonnewald & L. Willmitzer, 1992. Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes.EMBO Journal 11:1229–1238.

    PubMed  Google Scholar 

  • Pavia, E., R.M. Lister & W.D. Park, 1983. Induction and accumulation of major tuber proteins of potato in stems and petioles.Plant Physiology 71:161–168.

    Google Scholar 

  • Poovaiah, B.W., D. Takezawa, G. An & T.-J. Han, 1996. Regulated expression of a calmodulin isoform alters growth and development in potato.Journal of Plant Physiology 149:553–558.

    CAS  PubMed  Google Scholar 

  • Takezawa, D., Z.H. Liu, G. An & B.W. Poovaiah, 1995. Calmodulin gene family in potato: developmental and touch-induced expression of the mRNA encoding a novel isoform.Plant Molecular Biology 27:693–703.

    Article  CAS  PubMed  Google Scholar 

  • Taylor, M.A., H.V. Davies & L.A. Scobie, 1991. Molecular changes associated with tuberisation inSolanum tuberosum. Differential expression of α-tubulin isotypes.Physiologia Plantarum 81:244–250.

    Article  CAS  Google Scholar 

  • Taylor, M.A., A. Kumar, L.A. George & H.V. Davies, 1992a. Isolation and molecular characterisation of a tuberisation-related cDNA clone from potato (Solanum tuberosum L.).Plant Cell Reports 11:623–626.

    Article  CAS  Google Scholar 

  • Taylor, M.A., S.A. Mad Arif, A. Kumar, H.V. Davies, L.A. Scobie, S.R. Pearce & A.J. Flavell, 1992b. Expression and sequence analysis of cDNAs induced during the early stages of tuberisation in different organs of the potato plant (Solanum tuberosum L.).Plant Molecular Biology 20:641–651.

    Article  CAS  PubMed  Google Scholar 

  • Taylor, M.A., S.B. Smith, H.V. Davies & L.R. Burch, 1992c. The primary structure of a cDNA clone of the stearoyl-acyl carrier protein desaturase gene from potato (Solanum tuberosum L.).Plant Physiology 100:533–534.

    CAS  Google Scholar 

  • Vick, B.A. & D.C. Zimmerman, 1987. Oxidative systems for the modification of fatty acids. In: P.K. Stumpf & E.E. Conn (Eds), The Biochemistry of Plants, Vol. 9. Academic Press, New York, pp. 53–90.

    Google Scholar 

  • Visser, R.G.F., D. Vreugdenhil, T. Hendriks & E. Jacobsen, 1994. Gene expression and carbohydrate content during stolon to tuber transition in potatoes (Solanum tuberosum).Physiologia Plantarum 90:285–292.

    Article  CAS  Google Scholar 

  • Wang, W., D. Takezawa & B.W. Poovaiah, 1996. A potato cDNA encoding a homologue of mammalian multidrug resistant P-glycoprotein.Plant Molecular Biology 31:683–687.

    CAS  PubMed  Google Scholar 

  • Wu, H.-M., J. Zou, B. May, Q. Gu & A.Y. Cheung, 1993. A tobacco gene family for flower cell wall proteins with a proline-rich domain and a cystein-rich domain.Proceedings of the National Academy of Sciences of the United States of America 90:6829–6833.

    CAS  PubMed  Google Scholar 

  • Zheng, C.C., A.Q. Bui & S.D. Neill, 1993. Abundance of an mRNA encoding a high mobility group DNA-binding protein is regulated by light and an endogenous rhythm.Plant Molecular Biology 23:813–823.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unit of Plant Biochemistry, Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, Scotland, UK

    M. R. Macleod, H. V. Davies, Susan B. Jarvis & M. A. Taylor

Authors
  1. M. R. Macleod
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. V. Davies
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Susan B. Jarvis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Macleod, M.R., Davies, H.V., Jarvis, S.B. et al. Characterisation of genes isolated from a potato swellingstolon cDNA library. Potato Res 42, 31–42 (1999). https://doi.org/10.1007/BF02358389

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02358389

Additional keywords

Search

Navigation