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Cloning and Identification of Differentially Expressed Genes Associated with Smut in Sugarcane

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Abstract

The aim of this experiment was to evaluate the molecular mechanism of sugarcane response to the smut pathogen at the beginning of the pathogen infection of sugarcane seedlings, to explore related genes, and to provide useful information for developing rational strategies to control smut at early stages of disease development. A suppression subtractive hybridization library was constructed using cDNA synthesized from RNA extracted from normal stalks as driver and inoculated stalks as tester. The positive clones of the libraries were sequenced randomly, analyzed by BLAST, and classified by GO. A total of 248 positive clones were selected for sequencing, and a total of 224 EST sequences were obtained. In total, 188 ESTs were found to share a considerable homology with known genes, while the remaining 36 ESTs had no homology with known genes. In the Gene Ontology database, the unigenes were assigned functional descriptions; 152, 129, and 139 ESTs were, respectively, involved in cell component, molecular function, and biological process. Some genes related to a smut pathogen infection were obtained, while the SSH library was constructed. These genes reflected the regulation of sugarcane to smut pathogen and can be used as candidate genes.

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Funding

This article was supported by Guangxi Natural Science Fund (2015GXNSFBA139060), Guangxi Academy of Agricultural Sciences Fund (GNK 2016JZ01), Guangxi Key Laboratory of Sugarcane Genetic Improvement Fund (16-A-04-02), National 863 Projects of China (2013AA102604-01), Guangxi Funds for Bagui Scholars and Distinguished Experts (2013-03), and Fund for Guangxi Innovation Teams of Modern Agriculture Technology (gjnytxgxcxtd-03-01).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    Xiu-Peng Song, Zhen-Qiang Qin, Jin-Ju Wei, Chun-Yan Wei, Xiao-Qiu Zhang, De-Wei Li & Yang-Rui Li

  2. College of Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, China

    Ming-Hui Chen & Li-Tao Yang

  3. Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China

    Dan-Dan Tian

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  1. Xiu-Peng Song
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  2. Dan-Dan Tian
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  3. Ming-Hui Chen
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  4. Zhen-Qiang Qin
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  5. Jin-Ju Wei
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  9. Li-Tao Yang
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  10. Yang-Rui Li
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Correspondence to Li-Tao Yang or Yang-Rui Li.

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Song, XP., Tian, DD., Chen, MH. et al. Cloning and Identification of Differentially Expressed Genes Associated with Smut in Sugarcane. Sugar Tech 20, 717–724 (2018). https://doi.org/10.1007/s12355-018-0610-6

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  • DOI: https://doi.org/10.1007/s12355-018-0610-6

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