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Physical and functional mapping of rat-liver mitochondrial DNA

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Summary

Rat-liver mitochondrial DNA (mtDNA) contains 2 cleavage sites of the restriction endonuclease XbaI. The molecular sizes of restriction fragments are 6.6×106 and 3.7×106 D.

The results of partial cleavage of mtDNA with EcoRI allow the fragment F (0.32×106 D) to be localized in the sequence ABCEGFHDA. The functional map of mtDNA is constructed for two genes of the ATP-ase mRNAs from rat-liver mitochondria.

Molecular hybridization shows that the ATPase genes are located in fragment B and in the GEHD area of mtDNA EcoRI cleavage.

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Abbreviations

bp:

base pair(s)

rRNA:

ribosomal RNA

mRNA:

messenger RNA

SDS:

sodium dodecyl sulphate

D:

dalton

DEPC:

diethyl pyrocarbonate

MD:

megadalton

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Authors and Affiliations

  1. Institute of Experimental Medicine of the USSR Academy of Medical Sciences, Leningrad, USSR

    N. V. Tzimbalenko

  2. Institute of Molecular Biology of the USSR Academy of Sciences, Moscow, USSR

    V. V. Nosikov

Authors
  1. T. B. Kazakova
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  2. S. G. Babich
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  3. M. P. Mel'nikova
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  4. N. V. Tzimbalenko
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  5. E. A. Braga
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  6. V. V. Nosikov
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Kazakova, T.B., Babich, S.G., Mel'nikova, M.P. et al. Physical and functional mapping of rat-liver mitochondrial DNA. Mol Cell Biochem 35, 39–47 (1981). https://doi.org/10.1007/BF02358186

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  • DOI: https://doi.org/10.1007/BF02358186

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