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On heterogeneity of DNA methylases from Escherichia coli SK cells

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Summary

The presence ofE. coli SK cells of five different DNA-methylases differing in specificity to the methylated sequence is documented has been proven. Two enzymes methylate cytosine with the formation of 5′-methylcytosine and three enzymes methylate adenine with formation of 6′-methylaminopurine. A method for simultaneous isolation of the five individual enzymes including gel filtration on Biogel A-0.5 M is proposed. The direct evidence has been presented showing that the additional methylation test in our method modification actually can discriminate between enzymes differing in sensitive sites.

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Authors and Affiliations

  1. The Laboratory of Enzymology of the Academy of Medical Sciences of the USSR, Moscow, USSR

    I. I. Nikolskaya, N. G. Lopatina & S. S. Debov

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  1. I. I. Nikolskaya
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  2. N. G. Lopatina
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  3. S. S. Debov
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Nikolskaya, I.I., Lopatina, N.G. & Debov, S.S. On heterogeneity of DNA methylases from Escherichia coli SK cells. Mol Cell Biochem 35, 3–10 (1981). https://doi.org/10.1007/BF02358182

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  • DOI: https://doi.org/10.1007/BF02358182

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