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Cell culture aging

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Summary

Cellular research in aging has been stimulated by the observation that human diploid cells have a limited number of cell divisions in culture. This loss of cellular proliferation (in vitro senescence) has been extensively studied by biochemical, clonal, and genetic analysis. Studies of human skin fibroblast cultures have revealed thatin vitro senescense is related toin vivo human cellular aging. Recently differentiated cells have been proposed for aging studies. These cells may provide additional information on aging since alterations ofin vitro cellular functions may be related to thein vivo behavior of specific differented cell types.

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References

  1. Swim, H. E. & Parker, R. F., 1957. Am. J. Hyg. 66: 235–243.

    CAS  PubMed  Google Scholar 

  2. Hayflick, L. & Moorhead, P. S., 1961. Exp. Cell Res. 25: 585–621.

    Article  Google Scholar 

  3. Hayflick, L., 1965. Exp. Cell Res. 37: 614–636.

    Article  CAS  PubMed  Google Scholar 

  4. Moore, G. E., Gerner, R. E. & Franklin, H. A., 1967. J.A.M.A. 199: 87–92.

    Google Scholar 

  5. Dell'Orco, R. T., Mertens, J. G. & Kruse, P. F., Jr., 1974. Exp. Cell Res. 84: 363–366.

    PubMed  Google Scholar 

  6. Daniel, C. W., 1977. In: Handbook of the Biology of Aging (Finch, C. E. & Hayflick, L. eds.), pp. 122–158, Van Nostrand Reinhold, N.Y.

    Google Scholar 

  7. Cristofalo, V. J. & Scharf, B. B., 1973. Exp. Cell Res. 76: 419–427.

    Article  CAS  PubMed  Google Scholar 

  8. Maciera-Coelho, A., 1974. Nature 248: 421–422.

    Google Scholar 

  9. Simons, J. W. I. M., 1967. Exp. Cell Res. 45: 336–350.

    Article  CAS  PubMed  Google Scholar 

  10. Cristofalo, V. J. & Kritchevsky, D., 1969. Med. Exp. 19: 313–320.

    CAS  PubMed  Google Scholar 

  11. Macieria-Coelho, A. & Ponten, J., 1969. J. Cell Bio. 43: 374–377.

    Google Scholar 

  12. Bowman, P. D., Meek, R. L. & Daniel, C. W., 1975. Exp. Cell Res. 93: 184–190.

    Article  CAS  PubMed  Google Scholar 

  13. Mitsui, Y. & Schneider, E. L., 1976. Mech. Ageing Develop. 5: 45–56.

    Article  CAS  Google Scholar 

  14. Mitsui, Y. & Schneider, E. L., 1976. Exp. Cell Res. 100: 147–152.

    Article  CAS  PubMed  Google Scholar 

  15. Mitsui, Y. & Schneider, E. L., 1976. Exp. Cell Res. 103: 23–30.

    Article  CAS  PubMed  Google Scholar 

  16. Schneider, E. L. & Fowlkes, B. J., 1976. Exp. Cell Res. 98: 298–302.

    Article  CAS  PubMed  Google Scholar 

  17. Yanishevsky, R., Mendelson, M. L., Mayall, B. H. & Cristafalo, V. J., 1974. J. Cell Physiol. 84: 165–170.

    Article  CAS  PubMed  Google Scholar 

  18. Grove, G. L., Kress, E. D. & Cristofalo, V. J., 1976. J. Cell Biol. 70: 133a, Abstract 397.

    Google Scholar 

  19. Schneider, E. L. & Shorr, S. S., 1975. Cell 6: 179–184.

    Article  CAS  PubMed  Google Scholar 

  20. Cristofalo, V. J., Howard, B. V. & Kritchevsky, D., 1970. In: Organic, Biological and Medicinal Chemistry (Holeckova, E. & Cristafalo, V. J., eds.), pp. 83–119, Plenum Press, New York.

    Google Scholar 

  21. Wang, K. M., Rose, N. R., Bartholomew, E. A., Balzer, M., Berde, K. & Foldvosy, M., 1970. Exp. Cell Res. 61: 357–364.

    CAS  PubMed  Google Scholar 

  22. Hayflick, L., 1977. In: Handbook of the Biology of Aging (Finch, C. E. & Hayflick, L., eds.), pp. 159–186, Van Nostrand Reinhold, New York.

    Google Scholar 

  23. Hayflick, L., 1979. Mech. Ageing Dev. 9: 393–408.

    Article  CAS  PubMed  Google Scholar 

  24. Honda, S. & Matsuo, M., 1980. Mech. Ageing Dev. 12: 31–37.

    Article  CAS  PubMed  Google Scholar 

  25. Schactschable, D. O. & Wever, J., 1978. Mech. Ageing Dev. 8: 257–264.

    Google Scholar 

  26. Rosner, B. A. & Cristofalo, V. J., 1979. Mech. Ageing Dev. 9: 485–496.

    Article  CAS  PubMed  Google Scholar 

  27. Cristofalo, V. J., 1970. In: Aging in Cell and Tissue Culture (Cristofalo, V. S. & Holeckova, E., eds.), pp. 83–119, Plenum Press, New York.

    Google Scholar 

  28. Macieira-Coelho, A., 1966. Experientia 22: 390–391.

    Article  CAS  PubMed  Google Scholar 

  29. Rosner, B. A. & Cristofalo, V. J., 1979. Mech. Ageing Dev. 9: 485–496.

    Article  CAS  PubMed  Google Scholar 

  30. Smith, J. R. & Hayflick, L., 1974. J. Cell Biol. 62: 48–53.

    Article  CAS  PubMed  Google Scholar 

  31. Smith, J. R., Pereira-Smith, O. M. & Schneider, E. L., 1978. P.N.A.S. 75: 1353–1356.

    CAS  PubMed  Google Scholar 

  32. Smith, J. R. & Whitney, R. G., 1979. Science 207: 83–84.

    Google Scholar 

  33. Norwood, T. H., Pendergrass, W. R., Sprague, C. A. & Martin, G. M., 1974, P.N.A.S. 71: 2231–2235.

    CAS  PubMed  Google Scholar 

  34. Yanishevsky, R. M. & Stein, G. H., 1980. Exp. Cell Res. 126: 469–472.

    Article  CAS  PubMed  Google Scholar 

  35. Yanishevsky, R. & Carrano, A. V., 1975. Exp. Cell Res. 90: 169–174.

    Article  CAS  PubMed  Google Scholar 

  36. Norwood, T. H., Pendergrass, W. R. & Martin, G. M., 1975. J. Cell Biol. 64: 551–556.

    Article  CAS  PubMed  Google Scholar 

  37. Stein, G. H. & Yanishevsky, R. M., 1979. Exp. Cell Res. 120: 155–165.

    Article  CAS  PubMed  Google Scholar 

  38. Ranibovitch, P. S. & Norwood, T. H., 1979. J. Cell Biol. 83: 6A.

    Google Scholar 

  39. Stein, G. H. & Yanishevsky, R., 1980. J. Suparmolecular. Struc. Supp. 4: 152.

    Google Scholar 

  40. Littlefield, J. W., 1973. J. Cell Physiol. 82: 129–132.

    Article  CAS  PubMed  Google Scholar 

  41. Stanbridge, E. J., 1976. Nature 260: 17–20.

    Article  CAS  PubMed  Google Scholar 

  42. Wright, W. E. & Hayflick, L., 1975. Exp. Cell Res. 96: 113–121.

    CAS  PubMed  Google Scholar 

  43. Wright, W. E. & Hayflick, L., 1975. Fed. Proc. 34: 76–79.

    CAS  PubMed  Google Scholar 

  44. Muggleton-Harris, A. L. & Hayflick, L., 1976. Exp. Cell Res. 103: 321–330.

    CAS  PubMed  Google Scholar 

  45. Krohn, P. L., 1966. In: Topies in the Biology of Aging (Krohn, P. L., ed.), pp. 125–139, Wiley, New York.

    Google Scholar 

  46. Daniel, C. W., 1973. Experientia 29: 1422–1424.

    Article  CAS  PubMed  Google Scholar 

  47. Harrison, D. E., 1973. P.N.A.S. 70: 3184–3188.

    CAS  PubMed  Google Scholar 

  48. Schneider, E. L., 1979, Fed. Proc. 38: 1857–1861.

    CAS  PubMed  Google Scholar 

  49. Martin, G. M., Sprague, C. A. & Epstein, C. J., 1970. Lab. Invest 23: 86–92.

    CAS  PubMed  Google Scholar 

  50. Goldstein, S., Littlefield, J. W. & Soeldner, J. S., 1969. Proc. Natl. Acad. Sci. USA 64: 155–160.

    CAS  PubMed  Google Scholar 

  51. Schneider, E. L. & Witsui, Y., 1976. Proc. Natl. Acad. Sci. USA 73: 3584–3588.

    CAS  PubMed  Google Scholar 

  52. Orgel, L. E., 1963. Proc. Natl. Acad. Sci., USA 49: 517–521.

    CAS  PubMed  Google Scholar 

  53. Danner, D. D., Schneider, E. L. & Pitha, J., 1978. Exp. Cell Res. 114: 63–67.

    Article  CAS  PubMed  Google Scholar 

  54. Latt, S. A., 1973. Proc. Natl. Acad. Sci. USA 70: 3395–3399.

    CAS  PubMed  Google Scholar 

  55. Schneider, E. L., Chaillet, J. R. & Tice, R. R. 1976. Exp. Cell Res. 100: 396.

    Article  CAS  PubMed  Google Scholar 

  56. Perry, P. & Evan, H. J., 1975. Nature 258: 121–125.

    Article  CAS  PubMed  Google Scholar 

  57. Schneider, E. L. & Gilman, B., 1979. Hum Genet 46: 57–63.

    Article  CAS  PubMed  Google Scholar 

  58. Schneider, E. L. & Monticone, R. E., 1979. Exp. Cell. Res. 115: 269–276.

    Google Scholar 

  59. Kram, D., Schneider, E. L., Tice, R. & Gianas, P., 1978. Exp. Cell. Res. 114: 471–475.

    Article  CAS  PubMed  Google Scholar 

  60. Shock, N. W. & Morris, A., 1966. Ann. N.Y. Acad. Sci. 134: 591–601.

    Google Scholar 

  61. Tassin, J., Simonneau, L. & Courtois, Y., 1978. In: Biology of the Epithelial Lens Cells (Courtois, Y. & Regnault, F., eds.), pp. 145–162. Les Colloques de L'INSERM, Vol. 60. Paris.

  62. Perry, M. M., Tassin, J. & Courtois, Y., 1979. Exp. Eye. Res. 28: 327–341.

    Article  CAS  PubMed  Google Scholar 

  63. Rheinwald, J. G. & Green, H., 1975. Cell 6: 331–344.

    Article  CAS  PubMed  Google Scholar 

  64. Sun, T. T. & Green, H., 1976. Cell 9: 511–521.

    Article  CAS  PubMed  Google Scholar 

  65. Green, H. & Thomas, J., 1978. Science 200: 1385–1388.

    CAS  PubMed  Google Scholar 

  66. Ponten, J. & Macintyre, E. H., 1968. Acta Pathol. Microbiol. Scand. 74: 465–486.

    CAS  PubMed  Google Scholar 

  67. Fischbach, G. D. & Nelson, P. G., 1977. In: Handbook of Physiology Section 1. The Nervous System Volume I. Cellular Biology of Neurons, Part 2, American Physiological Society, Bethesda, Maryland, pp. 719–774.

    Google Scholar 

  68. McCarthy, K. D. & deVellis, J., 1980. J. Cell Biology 85: 890–902.

    Article  CAS  Google Scholar 

  69. Mueller, S. N., Rosen, E. M. & Levine, E. M., 1980. Science 207: 889–891.

    CAS  PubMed  Google Scholar 

  70. Ross, R. & Glomset, J. A., 1976. New Eng. J. Med. 295: 369–377 and 420–425.

    CAS  PubMed  Google Scholar 

  71. Bierman, E. L., 1978. In Vitro 14: 951–955.

    CAS  PubMed  Google Scholar 

  72. Bierman, E. L., Albers, J. J. & Chait, A., 1979. J. Geront. 34: 483–488.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. National Institute on Aging, NIH, 20205, Bethesda, MD, USA

    M. Reff & E. L. Schneider

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  1. M. Reff
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  2. E. L. Schneider
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Reff, M., Schneider, E.L. Cell culture aging. Mol Cell Biochem 36, 169–176 (1981). https://doi.org/10.1007/BF02357034

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  • DOI: https://doi.org/10.1007/BF02357034

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