Summary
The metabolism of calcium has been investigated in the Ehrlich Ascites Tumour Cells (ATC). ATC extrude Ca2+ actively by an energy-dependent mechanism, supported by both respiration and glycolysis.
Extrusion takes place even against a very steep concentration gradient (10 mM Ca2+).
Cell calcium content is decreased by monovalent cations (Na+, K+ and Li+), which act independently from their metabolic effects.
La3+ inhibits ATC Ca2+ extrusion whereas Ruthenium Red slightly decreases cell calcium content.
The antibiotic ionophore A 23187 strongly increases ATC Ca2+ level.
The metabolism of other divalent cations (Mg2+, Sr2+ and Mn2+) has been studied. Mg2+ does not show appreciable changes in the various metabolic conditions tested, while Mn2+ and Sr2+ behave quite differently from Ca2+, suggesting a different distribution of these cations in ATC.
The experimental findings indicate that Ehrlich Ascites Tumour Cells regulate their calcium content by mechanisms related to plasma membranes while the size and activity of mitochondrial compartment is of minor importance.
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References
Wenner, C. E., 1975. In: Cancer, A comprehensive treatise (Becker, F. F., ed.), Vol. 3, pp. 389–403, Plenum Press, New York.
Chance, B., 1965. J. Biol. Chem. 240:2729–2748.
Bygrave, F. L., 1966. Biochem. J. 101:480–487.
Loewenstein, W. R., 1966. In: Biological membranes recent progress (Lowenstein, W. R., ed), Ann. N. Y. Acad. Sci. 137: 441–472.
Bygrave, F. L., 1967. Nature 214:667–671.
Lehninger, A. L., Carafoli, E. & Rossi, S. C., 1967. In: Advances in enzymology (Nord, F. F., ed.), Vol. 29, pp. 259–320. Interscience, New York.
Smyth, H. & Flaharan E., 1969. Life Sci. 8: 1317–1322.
Balk, S. D., 1971. Proc. Natl. Acad. Sci. U.S. 68: 271–275.
Borle, A. B., 1973. Fed. Proc. 32: 1944–1950.
Schultz, G., Hardman, J. G., Hurwitz, L., & Sutherland, E. W., 1973. Fed. Proc. 32: 773–785.
Berridge, M. J., 1975. In: Advances in cyclic nucleotide research (Greengard, P. & Robinson, G. A., eds.), Vol. 6, pp. 1–98, Raven Press, New York.
Kolb, H. A. & Adam, G., 1976. J. Membrane Biol. 26: 121–151.
Boynton, A. L. & Whitfield, J. F. 1976. Proc. Natl. Acad. Sci. U.S. 73: 1651–1654.
Gilbert, I. G. F., 1972. Eur. J. Cancer 8: 99–105.
Bresciano, F. & Auricchio F., 1962. Cancer Res. 22: 1284–1289.
Anghileri, L. J., Miller, E. S., Robinette, J., Prasad, K. N. & Lagerbord, V. A., 1971. Oncology 25: 193–209.
Bygrave, F. L., 1978. Biol. Rev. 53: 43–79.
Cittadini, A., Scarpa, A. & Chance, B., 1971. FEBS Lett. 18: 98–102.
Cittadini, A., Scarpa, A. & Chance, B., 1973. Biochim. Biophys. Acta 291: 246–259.
Cittadini, A., Bossi, D., Rosi, G., Wolf, F. & Terranova, T., 1977. Biochim. Biophys. Acta 469: 345–349.
Dani, A. M., Cittadini, A., Calviello, G., Festuccia, G. & Terranova, T., 1978. Mol. Cell. Biochem. 22: 139–145.
Little, J. R., 1964. Anal. Biochem. 7: 87–95.
Szarkowska, L. & Klingenberg, M., 1963. Biochem. Z. 338: 674–697.
Mela, L., 1967. Fed. Proc. 26: 456.
Mela, L., 1968. Arch. Biochem. Biophys. 123: 286–293.
Pressman, B. C., 1976. Ann. Rev. Biochem. 45: 501–530.
Cittadini, A. & Rossum, G. D. V. van, 1978. J. Physiol. 281: 29–43.
Moore, C., 1971. Biochem. Biophys. Res. Commun. 42: 298–305.
Chance, B. & Mela, L., 1966. Biochemistry 5: 3220–3235.
Vainio, H., Mela, L. & Chance, B., 1970. Eur. J. Biochem. 12: 387–395.
Carafoli, E., 1967. J. Gen. Physiol. 50: 1849–1864.
Gunter, T. E., Gunter, K. K., Puskin, J. S. & Russel, P. R., 1978. Biochemistry 17: 339–345.
Rossum, G. D. V. van, 1970. J. Gen. Physiol. 55: 18–32.
Schatzman, H. J., 1975. In: Current topics in membrane and transport, Vol. 6, pp. 125–168, Academic Press, New York.
Rossum, G. D. V. van, Gosalvez, M., Galeotti, T. & Morris, H. P., 1971. Biochim. Biophys. Acta 245: 263–276.
Rossum, G. D. V. van, Smith, K. & Beton, P., 1976. Nature 260: 335–336.
Scarpa, A., Brinley, F. J. & Tiffert, T., 1978. Fed. Proc. 37: 1615.
Kotik, A. & Janacek, K., 1975. In: Cell membrane transport —Principles and techniques, 3nd ed., Plenum Press, New York.
Baker, P. F., 1976. In: Symposium XXX — Calcium in biological Systems (Duncan, C. J., ed.) pp. 67–88, Cambridge University Press.
Blaustein, M. P., 1974. Rev. Physiol. Biochem. Pharmacol. 70: 33–82.
Ash, G. R. & Bygrave, F. L., 1977. FEBS Lett. 78: 166–168.
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Cittadini, A., Bossi, D., Wolf, F. et al. Further observations on calcium and other divalent cations metabolism in intact Ehrlich ascites tumour cells. Mol Cell Biochem 36, 85–93 (1981). https://doi.org/10.1007/BF02354907
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DOI: https://doi.org/10.1007/BF02354907