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Sizes of granular vesicles in the rat median eminence, with special reference to the zona granulosa

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Summary

A quantitative evaluation has been made of the sizes of neuronal granular vesicles (GV) in different (sub-)zones of the rat median eminence. On the basis of the frequency distribution of the mean sizes of the GV in the individual nerve profiles, at least 4 tentative categories of nerve profiles may be distinguished. However, available data do not permit characterization of nerve fibre categories in terms of neurohormone and/or neurotransmitter storage.

From dorsal to ventral the palisade zone shows a decrease in mean size of GV; furthermore, the relative number of nerve profiles characterized by small GV (mean size smaller than 112 nm) increases. In contrast, the GV in the zona granulosa, that part of the external zone of the median eminence ventral to the palisade zone, are of relatively large size.

Qualitative observations indicate that the zona granulosa may be regarded as an area for both storage and release of biologically active substances.

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References

  • Aghajanian, G. K., Bloom, F. E.: Electron-microscopic localization of tritiated norepinephrine in rat brain: effect of drugs. J. Pharmacol. exp. Ther.156, 407–416 (1967)

    CAS  PubMed  Google Scholar 

  • Akmayev, I. G., Réthelyi, M., Majorossy, K.: Changes induced by adrenalectomy in nerve endings of the hypothalamic median eminence (zona palisadica) in the albino rat. Acta biol. Acad. Sci. hung.18, 187–200 (1967)

    CAS  PubMed  Google Scholar 

  • Barer, R., Lederis, K.: Ultrastructure of the rabbit neurohypophysis with special reference to the release of hormones. Z. Zellforsch.75, 201–239 (1966)

    Article  CAS  PubMed  Google Scholar 

  • Bloom, F. E., Aghajanian, G. K.: Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid. J. Ultrastruct. Res.22, 361–375 (1968)

    Article  CAS  PubMed  Google Scholar 

  • Cameron, E., Foster, C. L.: Some light- and electron-microscopical observations on the pars tuberalis of the pituitary gland of the rabbit. J. Endocr.54, 505–511 (1972)

    CAS  PubMed  Google Scholar 

  • Clementi, F., Ceccarelli, B., Cerati, E., Demonte, M. L., Pecile, A.: Subcellular organization of the median eminence. In: Advances in cytopharmacology (eds. F. Clementi, B. Ceccarelli), vol. 1, p. 331–342. New York: Raven Press 1971

    Google Scholar 

  • De Robertis, E.: Histophysiology of synapses and neurosecretion. Oxford: Pergamon Press 1964

    Google Scholar 

  • Doerr-Schott, J., Follenius, E.: Identification et localisation des fibres aminergiques dans l'éminence médiane de la Grenouille verte (Rana esculenta L.), par autoradiographie au microscope électronique. Z. Zellforsch.111, 427–436 (1970)

    CAS  PubMed  Google Scholar 

  • Douglas, W. W., Nagasawa, J., Schulz, R.: Electron microscopic studies on the mechanism of secretion of posterior pituitary hormones and significance of microvesicles (“synaptic vesicles”): evidence of secretion by exocytosis and formation of microvesicles as a byproduct of this process. In: Subcellular organization and function in endocrine tissue (eds. H. Heller, K. Lederis). Cambridge: University Press. Mem. Soc. Endocrinol.19, 353–378 (1971)

    Google Scholar 

  • Fink, G., Smith, G. C.: Ultrastructural features of the developing hypothalamo-hypophysal axis in the rat. Z. Zellforsch.119, 208–226 (1971)

    Article  CAS  PubMed  Google Scholar 

  • Hagen, E.: Über die feinere Histologic einiger Abschnitte des Zwischenhirns und der Neurohypophyse. Acta anat. (Basel)25, 1–33 (1955)

    CAS  Google Scholar 

  • Hanström, B.: The hypophysis in some south-african insectivora, carnivora, hyracoidea, proboscidea, artiodactyla, and primates. Ark. Zool.4, 187–294 (1952)

    Google Scholar 

  • Hökfelt, T.: The possible ultrastructural identification of tubero-infundibular dopamine containing nerve endings in the median eminence of the rat. Brain Res.5, 121–123 (1967)

    PubMed  Google Scholar 

  • Hökfelt, T.: In vitro studies on central and peripheral monoamine neurons at the ultrastructural level. Z. Zellforsch.91, 1–74 (1968)

    Article  PubMed  Google Scholar 

  • Hökfelt, T., Fuxe, K.: On the morphology and the neuroendocrine role of the hypothalamic catecholamine neurons. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich (eds. K. M. Knigge, D. E. Scott, A. Weindl), p. 181–223. Basel: Karger 1972

    Google Scholar 

  • Ishii, S.: Isolation and identification of secretory vesicles in the axons of the equine median eminence. Gunma Symp. Endocrinology,7, 1–11 (1970)

    Google Scholar 

  • Ishii, S.: Classification and identification of neurosecretory granules in the median eminence. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich (eds. K. M. Knigge, D. E. Scott, A. Weindl), p. 119–141. Basel: Karger 1972

    Google Scholar 

  • Kawashima, S., Hirano, T., Matsui, T., Takewaki, K.: Neurosecretion and neurohypophysial hormones in the rat median eminence after total hypophysectomy, with reference to histological and fine structure of the region. J. Fac. Sci,. Univ. Tokyo, sect.IV 2, 11–28 (1966)

    Google Scholar 

  • Kobayashi, H., Ishii, S.: The median eminence as storage site for releasing factors and other biologically active substances. Excerpta Mediaa Int. Congr. Ser.184, 548–554 (1968)

    Google Scholar 

  • Kobayashi, H., Matsui, T.: Fine structure of the median eminence and its functional significance. In: Frontiers in neuroendocrinology (eds. W. F. Ganong, L. Martini), p. 3–46. New York: Oxford University Press 1969

    Google Scholar 

  • Kobayashi, H., Matsui, T., Ishii, S.: Functional electron microscopy of the hypothalamic median eminence. Int. Rev. Cytol.29, 281–381 (1970)

    CAS  PubMed  Google Scholar 

  • Kobayashi, H., Oota, Y., Uemura, H., Hirano, T.: Electron microscopic and pharmacological studies on the rat median eminence. Z. Zellforsch.71, 387–404 (1966)

    Article  CAS  Google Scholar 

  • Kobayashi, T., Kobayashi, T., Yamamoto, K., Kaibara, M., Ajika, K.: Electron microscopic observation on the hypothalamo-hypophyseal system in the rat. III. Effect of reserpine treatment on the axonal inclusions in the median eminence. Endoer. jap.15, 321–335 (1968)

    CAS  Google Scholar 

  • Kobayashi, T., Kobayashi, T., Yamamoto, K., Kaibara, M., Ajika, K.: Electron microscopic observations of axonal inclusions in rat median eminence. Endoer. jap. suppl.1, 11–25 (1969)

    Google Scholar 

  • Kobayashi, T., Kobayashi, T., Yamamoto, K., Kaibara, M., Kameya, Y.: Electron microscopic observation on the hypothalamo-hypophyseal system in the rat II. Ultrafine structure of the median eminence and of the nerve cells of the arcuate nucleus. Endocr. jap.14, 158–177 (1967)

    CAS  Google Scholar 

  • Martinez, P.: The structure of the pituitary stalk and the innervation of the neuro-hypophysis in the cat. Diss. Leiden 1960

  • Matsui, T.: Fine structure of the median eminence of the rat. J. Fac. Sci, Tokyo, sect. IV,11, 71–96 (1966)

    Google Scholar 

  • Metuzals, J.: The structure of the hypothalamic final common path to the adeno-hypophysis in the cat. J. comp. Neurol.113, 103–138 (1959)

    Article  CAS  PubMed  Google Scholar 

  • Monroe, B. G.: A comparative study of the ultrastructure of the median eminence, infundibular stem and neural lobe of the hypophysis of the rat. Z. Zellforsch.76, 405–432 (1967)

    Article  CAS  PubMed  Google Scholar 

  • Monroe, B. G., Newman, B. L., Schapiro, S.: Ultrastructure of the median eminence of neonatal and adult rats. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich (eds. K. M. Knigge, D. E. Scott, A. Weindl), p. 7–26. Basel: Karger 1972

    Google Scholar 

  • Mulder, A. H., Geuze, J. J., Wied, D. de: Studies on the subcellular localization of corticotrophin releasing factor (CRF) and vasopressin in the median eminence of the rat. Endocrinology87, 61–79 (1970)

    CAS  PubMed  Google Scholar 

  • Nakai, Y., Shinkawa, Y.: Electron microscopic autoradiography on the localization of serotonin in the frog median eminence. Z. Zellforsch.119, 326–333 (1971)

    CAS  PubMed  Google Scholar 

  • Oota, Y., Kawabata, I., Kurosumi, K.: Electron microscopic studies on the rat hypothalamohypophyseal neurosecretory system. Jap. J. exp. Morph.20, 65–83 (1966). Citation by Ishii, 1972

    Google Scholar 

  • Palay, S. L.: The fine structure of the neurohypophysis. In: Ultrastructure and cellular chemistry of neural tissue (ed. H. Waelsch), p. 31–49. New York: Harper Hoeber 1957

    Google Scholar 

  • Peczely, P., Calas, A.: Ultrastructure de l'éminence médiane du pigeon (Columba livia domestics) dans diverses conditions expérimentales. Z. Zellforsch.111, 316–345 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Pellegrino de Iraldi, A., Jaim-Etcheverry, G.: Ultrastructural changes in the nerve endings of the median eminence after nialamide-DOPA administration. Brain Res.6, 614–618 (1967)

    Article  Google Scholar 

  • Raisman, G.: Electron microscopic studies of the development of new neurohaemal contacts in the median eminence of the rat after hypophysectomy. Brain Res.55, 245–261 (1973)

    Article  CAS  PubMed  Google Scholar 

  • Rinne, U. K.: Ultrastructure of the median eminence of the rat. Z. Zellforsch.74, 98–122 (1966)

    Article  CAS  PubMed  Google Scholar 

  • Rinne, U. K.: Experimental electron microscopic studies on the neurovascular link between the hypothalamus and anterior pituitary. In: Aspects of neuroendocrinology (eds. W. Bargmann, B. Scharrer), p. 220–228. Berlin-Heidelberg-New York: Springer 1970

    Google Scholar 

  • Rinne, U. K.: Effect of adrenalectomy on the ultrastructure and catecholamine fluorescence of the nerve endings in the median eminence of the rat. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich (eds. K. M. Knigge, D. E. Scott, A. Weindl), p. 164–170. Basel: Karger 1972

    Google Scholar 

  • Rinne, U. K., Sonninen, V., Helminen, H.: Ultrastructural alterations and changes in the catecholamine content of the neurosecretory nerve endings in the median eminence of the rat after oxypertine injection. Med. Pharmacol. exp.17, 108–118 (1967)

    CAS  Google Scholar 

  • Rodríguez, E. M.: Ultrastructure of the neurohaemal region of the toad median eminence. Z. Zellforsch.93, 182–212 (1969)

    PubMed  Google Scholar 

  • Rodríguez, E. M.: The comparative morphology of neural lobes of species with different neurohypophysial hormones. In: Subcellular organization and function in endocrine tissue (eds. H. Heller, K. Lederis). Cambridge: University Press. Mem. Soc. Endocrinol.19, 263–291 (1971)

    Google Scholar 

  • Rodríguez, E. M.: Comparative and functional morphology of the median eminence. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich (eds. K. M. Knigge, D. E. Scott, A. Weindl), p. 319–334. Basel: Karger 1972

    Google Scholar 

  • Röhlich, P., Vigh, B., Teichmann, I., Aros, B.: Electron microscopy of the median eminence of the rat. Acts biol. Acad. Sci. hung.15, 431–457 (1965)

    Google Scholar 

  • Scott, D. E., Knigge, K. M.: Ultrastructural changes in the median eminence of the rat following deafferentation of the basal hypothalamus. Z. Zellforsch.105, 1–32 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Stoeckart, R., Jansen, H. G., Kreike, A. J.: Ultrastructural evidence for exocytosis in the median eminence of the rat. Z. Zellforsch.131, 99–107 (1972)

    Article  CAS  PubMed  Google Scholar 

  • Stoeckart, R., Jansen, H. G., Kreike, A. J.: Quantitative data on the fine structural organization of the palisade zone of the median eminence. Z. Zellforsch.136, 111–120 (1973)

    Article  CAS  PubMed  Google Scholar 

  • Streefkerk, J. G.: Functional changes in the morphological appearance of the hypothalamohypophyseal neurosecretory and catecholaminergic neural system, and in the adenohypophysis of the rat. Diss. Amsterdam 1967

  • Unsicker, K.: On the innervation of mammalian endocrine glands (anterior pituitary and parathyroids). Z. Zellforsch.121, 283–291 (1971)

    Article  CAS  PubMed  Google Scholar 

  • Wendelaar Bonga, S. E.: Ultrastructure and histochemistry of neurosecretory cells and neurohaemal areas in the pond snailLymnaea stagnalis (L.). Z. Zellforsch.108, 190–224 (1970)

    CAS  PubMed  Google Scholar 

  • Wittkowski, W.: Kapillaren und perikapilläre Räume im Hypothalamus-Hypophysen-System und ihre Beziehungen zum Nervengewebe. Z. Zellforsch.81, 344–360 (1967)

    Article  CAS  PubMed  Google Scholar 

  • Wittkowski, W., Bock, R.: Electron microscopical studies of the median eminence following interference with the feedback system anterior pituitary adrenal cortex. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich (eds. K. M. Knigge, D. E. Scott, A. Weindl), p. 171–180. Basel: Karger 1972

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Erasmus University Rotterdam, P. O. Box 1738, Rotterdam, The Netherlands

    R. Stoeckart, A. J. Kreike & H. G. Jansen

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  1. R. Stoeckart
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  2. A. J. Kreike
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  3. H. G. Jansen
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We are grateful to Mrs. R.M.Y. Hartsteen for technical assistance, to Miss P.C. Delfos and Mr. W. van den Oudenalder for photographic assistance and to Prof. J. Moll for helpful criticism.

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Stoeckart, R., Kreike, A.J. & Jansen, H.G. Sizes of granular vesicles in the rat median eminence, with special reference to the zona granulosa. Z.Zellforsch 146, 501–515 (1973). https://doi.org/10.1007/BF02347179

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