Abstract
The aim of the present study was to evaluate the impact of prenatal exposure to low doses of aldrin on physical and behavioral developments of rats (1–21 days old). To detect the possible persistent adversities produced by this exposure, the animals were also tested when adults (90 days old). Plasma determinations of both aldrin and its metabolite dieldrin and histopathological evaluations of brain slices were also performed in adult animals. Pregnant female rats were subcutaneously (s. c.) treated with aldrin (1.0 mg/kg) or with its vehicle (0.9% NaCl solution plus Tween-80) from day 1 of pregnancy until delivery. Results show that prenatal aldrin administration was able to decrease the median effective time (TE50) for incisor teeth eruption and to increase the TE50 for testes descent; other parameters indicative of physical development were not modified. Aldrin and dieldrin were not found in plasma of the adult rats; no differences were observed between control and experimental rats in the cellular and structural organization of the cerebral cortex neurones. Prenatal aldrin administration produced no impairment of adult animal's behavior in an avoidance learning test; nevertheless, the locomotor frequency of the experimental rats was higher than that of controls at 21 and 90 days old. When adults, these experimental rats had their performance in a hole-board apparatus (total number and duration of headdips) also higher than that of the control ones. It was concluded that prenatal aldrin exposure induced not only developmental changes in the rat pups but also persistent behavioral alterations in adulthood, when the pesticide was not present in these animals.
References
Acs A, Kacsoh B, Veress Z, Toth BE (1988) Rat milk stimulates pituitary growth secretion of neonatal pituitaries in vivo. Life Sci 42: 2315–2321
Alder S (1983) Behavioral teratology. In: Zbinden G, Racagni G. Cuomo V, Weiss B (eds) Application of Behavioral Pharmacology in Toxicology. Raven Press, New York, pp 57–66
Bardo M, Bowling S, Pierce R (1990) Changes in locomotion and dopa mine neurotransmission following amphetamine haloperidol, and exposure to novel environmental stimuli. Psychopharmacology, 101: 338–343
Bernardi MM, Gaviolle MC, Spinosa HS, Palermo-Neto J, Castro VL Sinhorini I (1988) Significado anatomopatológico dos nívels plasmáticos de aldrin. Ars Veterinária 4 [1]: 125–134
Broadhurst P (1960) Experiments in psychogenetics. In: Eisenk E (ed) Experiments in Personality. Routlidge and Kegan Paul, London
Castro VL, Palermo-Neto J (1988) Alterations in the behavior of young and adult rats exposed to aldrin during lactation. Braz J Med Biol Res 21: 987–990
Castro VL, Palermo-Neto J (1989) Effects of a specific stress (aldrin) on rat progeny behavior. Braz J Med Biol Res 22: 979–982
Cornwell-Jones C, Decker M, Chang J, Cole B, Goltz K, Tran T. McGaugh J (1989) Neonatal 6-hydroxydopa but not DSP-4, elevates brainstem monoamines and impairs inhibitory avoidance learning in developing rats. Brain Res 493: 258–268
Dale W, Miles J (1970) Quantitative method for determination of DDT and DDT-metabolites in blood serum. JAOAC 53: 1287–1292
Dean M, Smeaton T, Stock B (1980) The influence of fetal and neonatal exposure to dichlorodiphenyltrichloroethane (DDT) on the testosterone status of neonatal male rat. Toxicol Appl Pharmacol 53: 315–322
Deichmann W, MacDonald W, Cubit D (1975) Dieldrin and DDT in the tissues of mice fed aldrin and DDT for seven generations. Arch Toxicol 34: 173–182
Eliason B, Posner H (1971) Placental passage of14C-dieldrin altered by gestational age and plasma proteins. Am J Obstet Gynecol 111: 925–929
File S, Wardill A (1985) The reliability of the hole-board apparatus. Psychopharmacologia 44: 47–51
Gray Jr L, Kavlock Jr R, Chernoff N, Gray J, McLamb J (1981) Perinatal toxicity of endrin in rodents III alterations of behavioral ontogeny Toxicology 21: 187–202
Gupta D (1983) Hormones and human milk. Endocrinol Exp (Bratisl) 17: 359–370
Johnson N, Leone F (1974) Statistics and experimental dosing. In: Engineering and physical sciences. John Wiley, New York, p 241–244
Koldovsky D, Bedrick A, Thornburg W (1986) Processing of hormonelike substances from milk in the gastrointestinal tract of suckling rats. Endocrinol Exp (Bratisl) 20: 119–130
Litchfield J (1949) A method for rapid graphic solution of time — per cent effect curves. J Pharmacol Exp Ther 97: 399–408
Lorenzim C, Bucherelli I, Giachetti A (1986) Some factors influencing conditioned and spontaneous behavior of rats in the light-dark box test. Physiol Behav 36: 97–101
Nasello A, Felício L (1988) Acute bromopride treatments: effects on general activity and inhibitory avoidance in rats. Braz J Med Biol Res 21: 841–843
Norton S (1982) Behavior versus morphology as indicator of central nervous system toxicity. In: Mitchell C (ed) Nervous system toxicology. Raven Press, New York
Spiegel M (1972) Statistics. Schaun Publishing Co., New York
Spyker DA, Spyker JM (1977) Response model analysis for cross-fostering studies prenatal versus postnatal effects on offspring exposed to methyl mercury dicyandiamide. Toxicol Appl Pharmacol 40: 511–527
Spyker J (1975) Assessing the impact of low level chemicals on development: behavioral and latent effects. Fed Proc 34: 1835–1844
Tilson H, Shaw S, McLamb R (1987) The effects of lindane, DDT and chlordecone on avoidance responding and seizure activity. Toxicol Appl Pharmacol 88: 57–65
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Castro, V.L., Bernardi, M.M. & Palermo-Neto, J. Evaluation of prenatal aldrin intoxication in rats. Arch Toxicol 66, 149–152 (1992). https://doi.org/10.1007/BF02342511
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DOI: https://doi.org/10.1007/BF02342511