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Localization of the mRNA encoding the zona protein ZP3α in the porcine ovary, oocyte and embryo by non-radioactivein situ hybridization

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Summary

The mRNA of the zona pellucida glycoprotein ZP3α was localized in frozen sections of pig ovaries, isolated oocytes and early embryos byin situ hybridization using biotinylated oligonucleotide probes. In follicles, the distribution of mRNA for ZP3α was correlated with the developmental stage: in primordial and primary follicles, the mRNA was shown to be predominantly localized in the oocyte. In secondary follicles, mRNA was found in both the oocyte and follicle cells. In tertiary and preovulatory follicles, the follicle cells showed distinct staining, whereas the oocyte was labelled weakly. In the early embryo, i.e. 2 days after fertilization, mRNA for ZP3α could not be demonstrated. Our results suggest that, in the pig, the zona pellucida protein ZP3α is synthesized by the oocyte and the follicle cells in sequence. After fertilization, synthesis of ZP3α is terminated.

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  1. Department of Veterinary Anatomy, University of Munich, Veterinärstrasse 13, 80539, Munich, Germany

    Sabine Kölle, Fred Sinowatz, Gudrun Boie, Ines Totzauer, Werner Amselgruber & Johnna Plendl

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  1. Sabine Kölle
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  2. Fred Sinowatz
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  5. Werner Amselgruber
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Kölle, S., Sinowatz, F., Boie, G. et al. Localization of the mRNA encoding the zona protein ZP3α in the porcine ovary, oocyte and embryo by non-radioactivein situ hybridization. Histochem J 28, 441–447 (1996). https://doi.org/10.1007/BF02331435

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  • DOI: https://doi.org/10.1007/BF02331435

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