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Plant regeneration from somatic embryos in black pepper

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Abstract

Embryogenic callus derived from zygotic embryos of black pepper (Piper nigrum Linn.) were induced to form somatic embryos on solid and liquid Schenk and Hildebrandt basal medium. Callus proliferation, somatic embryo-genesis and germination of embryos were achieved in about 8 months in static cultures while it took only 8 weeks in liquid suspension cultures. The highest number of embryos and plantlets was produced from cells grown as suspension cultures raised in half-strength medium without growth regulators and sucrose level reduced from 3% to 1.5%. Regenerated plants were established in soil.

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Abbreviations

2,4-d :

2,4-dichlorophenoxy acetic acid

SH:

Schenk and Hildebrandt

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Authors and Affiliations

  1. Department of Biotechnology, Calicut University, 673 635, Kerala, India

    Biju Joseph, Dominic Joseph & V. J. Philip

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  1. Biju Joseph
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  2. Dominic Joseph
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  3. V. J. Philip
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Joseph, B., Joseph, D. & Philip, V.J. Plant regeneration from somatic embryos in black pepper. Plant Cell Tiss Organ Cult 47, 87–90 (1996). https://doi.org/10.1007/BF02318970

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  • DOI: https://doi.org/10.1007/BF02318970

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