Abstract
A method is described for producing genetically transformed plants from explants of three scentedPelargonium spp. Transgenic hairy root lines were developed fromPelargonium spp leaf explants and microcuttings after inoculation withAgrobacterium rhizogenes strains derived from the agropine A4 strain. Hairy root lines grew prolifically on growth regulator-free medium. Transgenic shoots were regenerated from hairy roots and the plants have been successfully transferred to soil. The phenotype of regenerated plants has been characterized as having abundant root development, more leaves and internodes than the controls, short internodes and highly branched roots and aerial parts. Southern blot analyses have confirmed the transgenic nature of these plants.
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References
Ackermann C (1977) Pflanzen ausAgrobacterium rhizogenes tumoren aus Nicotiana tabacum. Plant Sci. Lett. 8: 23–30
Bevan M Chilton M (1982) T-DNA ofAgrobacterium Ti and Ri plasmids. Annu Rev Genet 16: 357–384
Cardarelli M, Mariotti D, Pomponi M, Spano L, Capone I & Costantino P (1987)Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype. Mol. Gen. Genet. 209: 475–480
Durand-Tardif M, Broglie R, Slightom J & Tepfer D (1985) Structure and expression of Ri T-DNA fromAgrobacterium rhizogenes inNicotiana tabacum: organ and phenotypic specificity. J. Mol. Biol 186: 557–564
Estruch J, Chriqui D, Grossmann K, Schell J & Spena A (1991) The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. EMBO J. 10: 2889–2895
Lambert C & Tepfer D (1992) Use ofAgrobacterium rhizogenes to create transgenic apple trees having an altered organogenic response to hormones. Theor. Appl. Genet 85: 105–109
Maniatis (1989) Molecular Cloning. Eds. Sambrook, Fritsh and Maniatis. Cold Spring Harbor Laboratory Press.
Martin-Tanguy J & Tepfer D (1990) Effects of Ri T-DNA fromAgrobacterium rhizogenes on growth, development and polyamine metabolism in tobacco. In H Flores, R Arteca, J Shannon, eds, Polyamines and Ethylene: Biochemistry, Physiology, and Interactions. American Society of Plant Physiologists, Rockville, pp 131–144
Maurel C, Barbierbrygoo H, Spena A, Tempe J & Guern J (1991) Single rol genes from theAgrobacterium rhizogenes Tl-DNA alter some of the cellular responses to auxin inNicotiana tabacum. Plant Physiol 97: 212–216
Michael A, Burtin D, Martin-Tanguy J & Tepfer D (1990) Effects of the rolC locus from the Ri TL-DNA ofAgrobacterium rhizogenes on development and polyamine metabolism in tobacco. In H Helsot, J Davis, J Florint, L Bobichon, G Durand, L Pénasse, eds, GIM 90 Proceedings. Société Française de Microbiologie, Paris, pp 863–868
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Pellegrineschi A, Damon J, Valtorta N, Paillard N & Tepfer D (1994) Improvement of ornamental characters and fragrance production in lemon-scented geranium through genetic transformation byAgrobacterium rhizogenes. Bio/technology 12 (Jan 1994): 64–68
Petit A, David C, Dahl G, Ellis J, Guyon P, Casse-Delbart F & Tempé J (1983) Further extension of the opine concept: plasmids inAgrobacterium rhizogenes co-operate for opine degradation. Mol. Gen. Genet. 190: 204–214
Rugini E, Pellegrineschi A, Mencuccini M & Mariotti D (1991) Increase of rooting ability in the woody species kiwi (Actinidia deliciosa a Chev) by transformation withAgrobacterium rhizogenes Rol Genes. Plant Cell Rep. 10: 291–295
Schmülling T, Schell J & Spena A (1988) Single genes fromAgrobacterium rhizogenes influence plant development. EMBO J. 7:2621–2629
Sinkar V, Pythoud F, White F, Nester E & Gordon M (1988) rol A locus of the Ri plasmid directs developmental abnormalities in transgenic plants. Genes and Dev. 2: 688–698
Slightom J, Durand-Tardif M, Jouanin L & Tepfer D (1986) Nucleotide sequence analysis of TL-DNA ofAgrobacterium rhizogenes agropine type plasmid. J. Biol. Chem. 261: 108–121
Tepfer D (1982) La transformation génétique de plantes supérieures parAgrobacterium rhizogenes. In eds, 2e Colloque sur les Recherches Fruitières. Centre Technique Interprofessionnel des Fruits et Légumes, Bordeaux, pp 47–59
Tepfer D (1983) The biology of genetic transformation of higher plants byAgrobacterium rhizogenes. In A Puhler, eds, Molecular genetics of the bacteria plant interaction. Springer Verlag, Berlin, pp 248–258
Tepfer D (1990) Genetic transformation usingAgrobacterium rhizogenes. Physiol. Plant. 79: 140–146
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Pellegrineschi, A., Davolio-Mariani, O. Agrobacterium rhizogenes-mediated transformation of scented geranium. Plant Cell Tiss Organ Cult 47, 79–86 (1996). https://doi.org/10.1007/BF02318969
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DOI: https://doi.org/10.1007/BF02318969