Summary
Plants were found to contain ecdysteroids in concentrations in the order of 0.1% which are higher than those of the insects, and are adequate for the preparative production of ecdysteroids.
A TLC screening method is elaborated for the qualitative and semiquantitative analysis of the ecdysteroid content of plants. The combined TLC method differentiates several characteristic ecdysteroids ranging from the apolar 2-deoxyecdysone to the polar 20,26-dihydroxyecdysone. A preparative purification method was developed consisting of solvent extraction, precipitation, crystallization and column liquid chromatography; these procedures permit the isolation of the main ecdysteroids in gram quantities. In some cases, droplet counter-current chromatography was also applied.
Altogether, the isolation of thirteen ecdysteroids from five different plant species was accomplished by using a fast, simple and low-cost procedure, resulting in pure substances.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. Báthori, I. Tóth, K. Szendrei, I. Reisch, Phytochemistry,21, 236–238 (1982).
M. Báthori, I. Tóth, K. Szendrei, M. Rattai, E. Minker, G. Blazsó, Herba Hungarica23, 131–143 (1984).
M. Báthori, K. Szendrei, Herba Hungarica,21, 157–164 (1982).
M. Báthori, I. Tóth, K. Szendrei, E. Minker, G. Blazsó, Fitoterápia,52, 77–80 (1980).
M. Báthori, K. Szendrei, I. Herke, Herba Hungarica, in press.
E. Ohnishi, M. Takashi, F. Chatani, N. Ikekawa, S. Sakurai, Science,197, 66–67 (1977).
P. Maróy, I. Vargha, K. Horváth, FEBS Letters,81, 319–321 (1977).
D. H. S. Horn in: “Naturally Occurring Insecticides, 9”,M. Jakobson, D. G. Crosby, Eds., Marcel Dekker, Inc., New York, 1971; pp. 333–459.
M. N. Galbraith, D. H. S. Horn, Aust. J. Chem.,22, 1045–1057 (1969).
M. N. Galbraith, D. H. S. Horn, E. J. Meddleton, R. J. Hackney, Aust. J. Chem.22, 1059–1067 (1969).
P. Karison, in “Progress in Ecdysone Research”,J. A. Hoffmann, Ed., Elsevier, Amsterdam, 1980; pp. 1–11.
E. D. Morgan, C. F. Poole, Comp. Biochem. Physiol.,57, 99–109 (1977).
K. Nakanishi, Pure Appl. Chem.,25, 167–195 (1971).
D. H. S. Horn, S. Fabbri, F. Hampshire, M. E. Lowe, Biochem. J.,109, 399–406 (1968).
S. Imai, M. Hori, S. Fujioka, E. Murata, M. Goto, K. Nakanishi, Tetrahedron Letters, 3883–3886 (1968).
H. Mori, K. Shibata, K. Tsuneda, M. Sawao, Chem. Pharm. Bull.,16, 1593–1600 (1968).
C. Wei-shan, in “Progress in Ecdysone Research”,J. A. Hoffmann, Ed., Elsevier, Amsterdam, 1980; pp. 281–297.
C. Hetru, D. H. S. Horn, in “Progress in Ecdysone Research”,J. A. Hoffmann, Ed., Elsevier, Amsterdam, 1980; pp. 13–18.
M. B. Gorovic, J. K. Zacnii, N. K. Abubakirov, Rasztit. Resz.,10, 261–274 (1974).
K. Hostettmann, M. Hostettmann, K. Nakanishi, J. Chromatogr.,170, 355 (1979).
J. Kubo, J. A. Klocke, J. Gonijan, N. Ishikawa, T. Matsumoto, J. Chromatogr.,257, 157–161 (1983).
E. Stahl, Dünnschichtchromatographie, 2nd edition, Springer, Berlin, 1967; p. 854.
N. K. Abubakirov, Khim.Prirod. Soed.,6, 685–702 (1981).
J. A. Hoffmann, M. Lagneus, C. Hetm, M. Charlet, F. Goltzene, in “Progress in Ecdysone Research”,J. A. Hoffmann, Ed., Elsevier, Amsterdam, 1980; pp. 440–444.
E. Ohnishi, T. Mizuno, N. Ikakawa, T. Ikeda, Insect. Biochem.,11, 155–159 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Báthori, M., Szendrei, K. & Herke, I. Application of combined chromatographic techniques in the screening and purification of ecdysteroids. Chromatographia 21, 234–238 (1986). https://doi.org/10.1007/BF02311894
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02311894