Summary
Since its introduction around 1977, OPLC has found widespread application and its methodology has been further developed. A possible development is gradient elution. Different continuous development methods (e.g. with eluent changeover, multistep gradient) and the possible system configurations for their execution are discussed. The advantages of the newly developed continuous development techniques are compared to earlier data. The most versatile method is demonstrated on lipid samples derived from human plasma, giving separation of six lipid classes and intra-class separation of the most polar classes in the same run.
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References
E. Tyihák, H. Kalász, E. Mincsovics, in Proceedings of the 17th Annual Meeting of the Hungarian Biochemical Society (Kecskemét, 1977); p. 256.
H. Kalász, in Chromatography — the State of the ArtH. Kalász andL. S. Ettre, eds., Akadémiai Kiadó, Budapest, 1985, pp. 501–515.
E. Tyihák, T. J. Székely, E. Mincsovics, in Proceedings of the Second Internat. Symposium on Instrumental HPTLC (Interlaken, 1982),R. E. Kaiser, ed., Institute für Chromatographie, Bad Dürkheim, 1982, pp. 159–173.
M. F. Gonnord, G. Guiochon, in Abstracts of the International Symposium on TLC With Special Emphasis on Overpressured-Layer Chromatography (Szeged, 1984),H. Kalász, ed., REANAL. Budapest, 1984, p. 35.
J. Pick, J. Vajda, L. Leisztner, J. Liquid Chromatogr.7, 2759–2776 (1984).
J. Pick, J. Vajda, N. Anh-Tuan, L. Leisztner, S. R. Hollán, J. Liquid Chromatogr.7, 2777–2791 (1984).
J. Pick, J. Vajda, L. Leisztner, J. Liquid Chromatogr.6, 2647–2660 (1983).
R. Yu, R. W. Ledeen, J. Lipid Research13, 680–686 (1972).
L. Svennerholm, P. Fredman, Biochim. Biophys. Acta617, 97–109 (1980).
E. Tyihák, E. Mincsovics, H. Kalász, J. Chromatogr.174, 75–81 (1975).
L. Vajda, M. Vajda, Z. Malisák, Gy. Petrányi, in Proceedings of the International Symposium TLC With Special Emphasis on Overpressured-Layer Chromatography (Szeged, 1984),E. Tyihák ed., LABOR MIM, Esztergom, 1985, in press.
M. Gurkin, J. Ripphahn, Internat. Laboratory,10, (4), 63–69 (May/June 1980).
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Vajda, J., Leisztner, L., Pick, J. et al. Methodological developments in overpressured-layer chromatography. Chromatographia 21, 152–156 (1986). https://doi.org/10.1007/BF02311744
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DOI: https://doi.org/10.1007/BF02311744