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Mass overload in preparative liquid chromatography: Choice of column dimensions and optimum working conditions

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Summary

This paper deals with a theoretical study which makes it possible to calculate column dimensions (length and inner diameter) and to select working conditions (particle diameter of the column packing, elution flow rate, run number) with regard to purity, recovery ratio and amount of pure substance that is to be isolated. These determinations take into account the pressure limitation and pumping capacity of the available chromatograph.

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Abbreviations

Q:

amount of pure substance to be recovered

p:

purity

r:

recovery ratio

RH :

throughput

ΔPmax :

maximum pressure allowed

Dmax :

pumping capacity

L:

column length

dc :

column inner diameter

dp :

particle diameter of stationary phase

n:

number of repetitive injections

Qi :

injectable component load

ε:

total porosity

γi :

reduced injectable amount

N:

number of theoretical plates

K:

parameter of sample composition

S:

sample solubility in mobile phase

FK,r,p,S :

function defined by eq. (3)

tR, 2 :

retention time of solute 2

D:

elution flow rate

H:

height equivalent to a theoretical plate

u:

linear velocity of mobile phase

\(G_{d_p } \) :

function defined by eq. (7)

ΔP:

pressure drop

η:

viscosity of mobile phase

Ko :

permeability of packing

k ′2 :

capacity factor of solute 2

Vc :

cut elution volume

zc :

reduced cut elution volume

Vo :

injection volume

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique III, (U. A. 04.0435 M. Porthault), Université Claude Bernard, 43 boulevard du 11 Novembre, 69622, Lyon I, Villeurbanne Cedex, France

    G. Cretier & J. L. Rocca

Authors
  1. G. Cretier
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  2. J. L. Rocca
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Cretier, G., Rocca, J.L. Mass overload in preparative liquid chromatography: Choice of column dimensions and optimum working conditions. Chromatographia 21, 143–148 (1986). https://doi.org/10.1007/BF02311742

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  • DOI: https://doi.org/10.1007/BF02311742

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