Summary
The construction and operation of a liquid preparativescale continuous chromatograph is described. The chromatographic bed is arranged in a series of interconnected vertical columns and is rotated whilst one end of the tube bundle is closed by a stationary plate through which liquids enter and leave the bed. The problem of maintaining an efficient seal is described. The chromatograph was commissioned by continuously fractionating dextran on porous silica beads with water as eluent. Results show that as the feed concentration was increased, the fractionation obtained deviated markedly from that expected from basic GPC theory, but that the relative molecular mass distribution of the feed was still narrowed with feed concentrations of 20% w/v.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
P. E. Barker, B. W. Hatt andA. N. Williams, Chromatographia10, 377 (1977).
M. Rendell, Process Engng., April 1975, 66.
P. E. Barker, inE. S. Perry andC. J. Van Oss (Editors), Progress in Separation and Purification, Vol. 4, J Wiley and Sons Inc. New York, 1971, p. 325.
M. V. Sussman andR. N. S. Rathore, Chromatographia8, 55 (1975).
P. E. Barker andR. E. Deeble, Chromatographia8, 67 (1975).
P. E. Barker, inC. E. H. Knapman (Editor), Developments in Chromatography, Applied Science Publishers, London, 1978.
P. E. Barker, F. J. Ellison andB. W. Hatt, Chem. Soc. Lond. Symposium, Advances in Chromatographic Fractionation of Macromolecules, Birmingham, July 1976. Paper published in “Chromatography of Synthetic and Biological Polymers”. Vol. I, pages 218–238, published by Horwood Ltd., 1978.
P. E. Barker, F. J. Ellison andB. W. Hatt, to be published, I & EC Process Design & Development, 1978.
P. E. Barker, S. A. Barker, B. W. Hatt andP. J. Somers, Chem. Proc. Eng.52(1), 64 (1971).
C. J. Hooke, Dept. of Mechanical Engineering, University of Birmingham, private communication.
S. A. Barker, B. W. Hatt andP. J. Somers, Carbohyd. Res.11, 355 (1969).
S. A. Barker, B. W. Hatt, J. B. Marsters andP. J. Somers, Carbohyd. Res.9, 373 (1969).
K. Hellsing, J. Chromatog.36, 170 (1968).
T. C. Laurent, Acta Chem. Scand.17, 2664 (1963).
T. C. Laurent, Biochem. J.89, 253 (1963).
F. J. Ellison, Ph. D. Thesis, University of Aston in Birmingham, 1976.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Barker, P.E., Hatt, B.W. & Williams, A.N. Fractionation of a polymer using a preparative-scale continuous chromatograph. Chromatographia 11, 487–493 (1978). https://doi.org/10.1007/BF02311069
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02311069