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Electrophoretic patterns of seed globulins in the Old-WorldLupinus species

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Summary

Electrophoretic analysis of seed globulins was performed to investigate relationships between the Old-WorldLupinus species. The analysis covered 11 species described earlier and the recently reported taxon,Lupinus anatolicus nom. nud. With only two exceptions, particular species were represented by various accessions. In total, 110 accessions were examined. SDS-PAGE of reduced globulins was carried out in a discontinuous buffer system.

The results showed a marked difference between the smooth-seeded and the rough-seeded species. In the group of smooth-seeded lupins pronounced interspecific differences were recorded. The rough-seeded species formed a rather homogenous group, well distinguishable from the smooth-seeded species. Though no distinct interspecific differences were recorded in the rough-seeded group, most of the 34 investigatedL. atlanticus accessions could be distinguished due to a characteristic, prominent polypeptide band with approx. Mr 27 kDa. The recently reported smooth-seeded taxon,L. anatolicus, showed a species-specific SDS-PAGE globulin pattern.

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Abbreviations

AUS:

Australia

CBB G 250:

Coomassie Brilliant Blue G 250

DZA:

Algeria

EGY:

Egypt

ESP:

Spain

GRC:

Greece

ISR:

Israel

ITA:

Italy

KEN:

Kenya

2-Me:

2-mercaptoethanol

MAR:

Morocco

Mr:

relative molecular mass

PAGE:

polyacrylamide gel electrophoresis

POL:

Poland

PRT:

Portugal

SDS:

sodium dodecyl sulphate

SYR:

Syria

TUR:

Turkey

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Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479, Poznań, Poland

    Janina Przybylska & Zdzisława Zimniak-Przybylska

Authors
  1. Janina Przybylska
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  2. Zdzisława Zimniak-Przybylska
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Additional information

Some of the presented results were announced in the form of a poster at the VIIth International Lupin Conference, Evora, Portugal, April 18–23,1993.

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Przybylska, J., Zimniak-Przybylska, Z. Electrophoretic patterns of seed globulins in the Old-WorldLupinus species. Genet Resour Crop Evol 42, 69–75 (1995). https://doi.org/10.1007/BF02310686

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  • DOI: https://doi.org/10.1007/BF02310686

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