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Genetic Resources and Crop Evolution

, Volume 42, Issue 1, pp 69–75 | Cite as

Electrophoretic patterns of seed globulins in the Old-WorldLupinus species

  • Janina Przybylska
  • Zdzisława Zimniak-Przybylska
Article

Summary

Electrophoretic analysis of seed globulins was performed to investigate relationships between the Old-WorldLupinus species. The analysis covered 11 species described earlier and the recently reported taxon,Lupinus anatolicus nom. nud. With only two exceptions, particular species were represented by various accessions. In total, 110 accessions were examined. SDS-PAGE of reduced globulins was carried out in a discontinuous buffer system.

The results showed a marked difference between the smooth-seeded and the rough-seeded species. In the group of smooth-seeded lupins pronounced interspecific differences were recorded. The rough-seeded species formed a rather homogenous group, well distinguishable from the smooth-seeded species. Though no distinct interspecific differences were recorded in the rough-seeded group, most of the 34 investigatedL. atlanticus accessions could be distinguished due to a characteristic, prominent polypeptide band with approx. Mr 27 kDa. The recently reported smooth-seeded taxon,L. anatolicus, showed a species-specific SDS-PAGE globulin pattern.

Key words

Electrophoresis Lupinus species Old-World rough-seeded smooth-seeded seed globulins 

Abbreviations

AUS

Australia

CBB G 250

Coomassie Brilliant Blue G 250

DZA

Algeria

EGY

Egypt

ESP

Spain

GRC

Greece

ISR

Israel

ITA

Italy

KEN

Kenya

2-Me

2-mercaptoethanol

MAR

Morocco

Mr

relative molecular mass

PAGE

polyacrylamide gel electrophoresis

POL

Poland

PRT

Portugal

SDS

sodium dodecyl sulphate

SYR

Syria

TUR

Turkey

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Janina Przybylska
    • 1
  • Zdzisława Zimniak-Przybylska
    • 1
  1. 1.Institute of Plant GeneticsPolish Academy of SciencesPoznańPoland

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