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The rapid quantitative analysis of codeine phosphate drug substance by reversed-phase high-performance liquid chromatography

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Summary

A rapid procedure for the evaluation of codeine phosphate drug substance derived from poppy straw or opium concentrate is described. The gradient HPLC procedure employs a pH 2.5 phosphate buffer, methanol and acetonitrile mobile phase at 40°C and a μBondapak C-18 column. Eight opium alkaloids, including the four major alkaloids, are separated from each other. The paper describes details of the assay procedure and presents data documenting the linearity, specificity, precision, and sensitivity of the method.

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Authors and Affiliations

  1. Analytical Development Department, McNeil Pharmaceutical, 19477, Spring House, PA, USA

    W. R. Sisco, C. T. Rittenhouse & W. M. Maggio

Authors
  1. W. R. Sisco
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  2. C. T. Rittenhouse
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  3. W. M. Maggio
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Sisco, W.R., Rittenhouse, C.T. & Maggio, W.M. The rapid quantitative analysis of codeine phosphate drug substance by reversed-phase high-performance liquid chromatography. Chromatographia 20, 289–292 (1985). https://doi.org/10.1007/BF02310385

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  • DOI: https://doi.org/10.1007/BF02310385

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