Abstract
Glucose metabolism has been studied in two strains ofAcinetobacter calcoaceticus. Strain LMD 82.3, was able to grow on glucose and possessed glucose dehydrogenase (EC 1.1.99.17). Glucose oxidation by whole cells was stimulated by PQQ, the prosthetic group of glucose dehydrogenase. PQQ not only increased the rate of glucose oxidation and gluconic acid production but also shortened the lag phase for growth on glucose.
Strain LMD 79.41 also possessed glucose dehydrogenase but was unable to grow on glucose. Batch cultures and carbon-limited chemostat cultures growing on acetate in the presence of glucose oxidized the sugar to gluconic acid, which was not further metabolized. However, after prolonged cultivation on mixtures of acetate and glucose, carbon-limited chemostat cultures suddenly acquired the capacity to utilize gluconate. This phenomenon was accompanied by the appearance of gluconate kinase and a repression of isocitrate lyase synthesis. In contrast to the starter culture, cells from chemostats which had been fully adapted to gluconate utilization, were able to utilize glucose as a sole carbon and energy source in liquid and solid media.
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References
Baumann P, Doudoroff M & Stanier RY (1968) A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J. Bacteriol. 95: 1520–1541
Clarke PH (1984) Evolution of new phenotypes. In: Klug MJ & Reddy CA (Eds) Current Perspectives in Microbial Ecology (pp 71–78) American Society of Microbiology, Washington D.C.
Dixon GH & Kornberg HL (1959) Assay methods for key enzymes of the glyoxylate cycle. Biochem. J. 72: 3
Duine JA, Frank J Jzn & Jongejan JA (1986) PQQ and quinoprotein enzymes in microbial oxidations. FEMS Microbiol. Rev. 32: 165–178
Hall BG (1984) Adaptation by acquisition of novel enzyme activities in the laboratory. In: Klug MJ & Reddy CA (Eds) Current Perspectives in Microbial Ecology (pp 79–86) American Society of Microbiology, Washington D.C.
Juni E (1972) Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J. Bacteriol. 112: 917–931
Juni E (1978) Genetics and physiology ofAcinetobacter. Ann. Rev. Microbiol. 32: 349–371
Juni E (1984) In: Krieg NR & Holt JG (Eds) Bergey's Manual of Systematic Bacteriology (pp 303–307) William & Wilkins, Baltimore London
Kaplan N & Rosenberg E (1982) Exopolysaccharide distribution of and bioemulsifier production byAcinetobacter calcoaceticus BD4 and BD 413. Appl. Environ. Microbiol. 44: 1335–1341
Kersters K & De Ley J (1984) In: Krieg NR & Holt JG (Eds) Bergey's Manual of Systematic Bacteriology. Vol. 1. (pp 361–373) Williams & Wilkins, Baltimore London
Kleber HP, Haferburg D, Asperger O, Schmidt M & Aurich H (1984) Aufnahme und Oxidation von Monosacchariden beiAcinetobacter calcoaceticus. Z. Allg. Mikrobiol. 24: 691–701
Lessie TG & Phibbs PV Jr (1984) Alternative pathways of carbohydrate utilization in pseudomonads. Ann. Rev. Microbiol. 38: 359–387
Mortlock RP (1982) Regulatory mutations and the development of new metabolic pathways by bacteria. In: Hecht, MK, Wallace B & Prince GT (Eds) Evolutionary Biology. Vol. 14 (pp 205–268) Plenum Press New York
Palleroni NJ (1984) In: Krieg NR & Holt JG (Eds) Bergey's Manual of Systematic Bacteriology. Vol. 1. (pp 141–149) Williams & Wilkins, Baltimore London
Schie van BJ, Dijken van JP & Kuenen JG (1984) Non-coordinated synthesis of glucose dehydrogenase and its prosthetic group PQQ inAcinetobacter andPseudomonas species. FEMS Microbiol. Lett. 24: 133–138
Schie van BJ, Hellingwerf KJ, Dijken van JP, Elferink MGL, Dijl van JMJG, Kuenen JG & Konings WN (1985). Energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase inEscherichia coli, Pseudomonas aeruginosa andAcinetobacter calcoaceticus (var. Iwoffi). J. Bateriol. 163: 493–499
Schie van BJ, Mooy de OH, Linton JD, Dijken van JP & Kuenen JG (1987a) PQQ-dependent production of gluconic acid byAcinetobacter, Agrobacterium andRhizobium species. J. Gen. Microbiol. 133: 867–875
Schie van BJ, Rouwenhorst RJ, Bont de JAM, Dijken van JP & Kuenen JG (1987b). an in vivo analysis of the energetics of aldose oxidation byAcinetobacter calcoaceticus. Appl. Microbiol. Biotechnol. 26: 560–567
Schie van BJ, Dijken van JP & Kuenen JG (1988) Effects of growth rate and oxygen tension on glucose dehydrogenase activity inAcinetobacter calcoaceticus LMD 79.41. Antonie van Leeuwenhoek 55: 53–65 (this issue)
Szymona M & Doudoroff M (1960) Carbohydrate metabolism inRhodopseudomonas spheroides. J. Gen. Microbiol. 22: 167–183
Taylor WH & Juni E (1961) Pathways for biosynthesis of a bacterial capsular polysaccharide. J. Bacteriol. 81: 694–703
Zwaig N, Nagel-de Zwaig R, isturiz T & Wecksler M (1973) Regulatory mutations affecting the gluconate system inEscherichia coli. J. Bacteriol. 114: 469–473
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Van Schie, B.J., Rouwenhorst, R.J., Van Dijken, J.P. et al. Selection of glucose-assimilating variants ofAcinetobacter calcoaceticus LMD 79.41 in chemostat culture. Antonie van Leeuwenhoek 55, 39–52 (1989). https://doi.org/10.1007/BF02309618
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DOI: https://doi.org/10.1007/BF02309618