Abstract
In adult albino rabbits, the influence of corticosteroids on the regeneration of the corneal endothelium after cryocoagulation was studied using light-, scanning- and transmission electron microscopy. The follow-up period varied from 10 h to 10 days. In the regenerating endothelium, both mitotic and amitotic cells division were seen. In the dexamethasone-treated eyes, the regeneration process seemed to be stimulated rather than inhibited. In five of six eyes, areas of hyperproliferation were found, consisting of numerous elongated cells that formed several cell layers. Most of these cells revealed an extensively developed endoplasmic reticulum and Golgi apparatus as well as numerous mitochondria. There were signs of secretory activity. The mechanism for the development of these multilayered hyperproliferation zones is unknown.
Zusammenfassung
Der Einfluß von Corticosteroiden auf die Regeneration des cornealen Endothels nach Kryokoagulation wurde an erwachsenen Albinokaninchen mittels Licht-, Raster- und Transmissionselektronenmikroskopie untersucht. Der Regenerationsprozeß der mit Dexamethason behandelten Augen war eher stimuliert als gehemmt. 3–10 Tage nach Kryoläsion konnten in 5 von 6 Augen Hyperproliferationsflächen beobachtet werden. Diese hyperproliferativen Zonen bestanden aus länglichen Zellen, die mehrere Schichten übereinander auf der Descemet'schen Membran bildeten. Diese Zellen entwickelten ein ausgedehntes ER, Golgi-Apparate und zahlreiche Mitochondrien. Auffallend war eine sekretorische Aktivität der Zellen. Der Mechanismus der Entwicklung dieser vielschichtigen Hyperproliferationszonen ist unbekannt.
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This study was kindly supported by the Deutsche Forschungsgemeinschaft (Grant Ro 81-18/2)
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Enke, P., Rohen, J.W. Morphological studies on the regeneration of rabbit corneal endothelium under the influence of corticosteroids. Graefe's Arch Clin Exp Ophthalmol 220, 19–24 (1983). https://doi.org/10.1007/BF02307011
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DOI: https://doi.org/10.1007/BF02307011