Summary
An organically modified silica glass material containing octyl groups (C8) was fabricated by the sol-gel process and coated onto the inner walls of fused silica capillaries. The coating was used as the stationary phase for reversed-phase open tubular liquid chromatography (OTLC). The sol-gel process facilitated the fabrication of a porous silica glass layer with the stationary phase in one single step. The high surface area of the coating, characteristic of materials fabricated by the sol-gel process, resulted in capillary columns with a stronger retention than those prepared by a conventional procedure. The molar ratio of the precursors was used as a tunable parameter to adjust the retention and selectivity of the stationary phase. The column performance was evaluated using anthracene derivatives as model analytes which were monitored by laser-induced fluorescence (LIF) detection. Separation efficiencies of 200,000 theoretical plates/meter were typical for the model compounds. Solute selectivity of the sol-gel derived stationary phase was found to be similar to the selectivity of HPLC monomeric-type phases.
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References
J. G. Dorsey, W. T. Cooper, J. F. Wheeler, H. G. Barth, J. P. Foley, Anal. Chem.66, 531R (1994).
R. T. Kennedy, M. D. Oates, B. R. Cooper, B. Nickerson, J. W. Jorgenson, Science246, 57 (1989).
M. D. Oates, J. W. Jorgenson, Anal. Chem.62, 1577 (1990).
J. H. Knox, M. T. Gilbert, J. Chromatogr.186, 405 (1979).
J. W. Jorgenson, E. J. Guthrie, J. Chromatogr.255, 335 (1983).
D. Ishii, T. Takeuchi, J. Chromatogr. Sci.18, 462 (1980).
H. A. Claessens, M. J. J. Hetem, P. A. Leclercq, C. A. Cramers, J. High Resolut. Chromatogr. Chromatogr. Commun.11, 176 (1988).
M. Novotny, Anal. Chem.60, 500A (1988).
A. Manz, W. Simon, J. Chromatogr.387, 187 (1987).
R. Swart, J. C. Kraak, H. Poppe, J. Chromatogr. A689, 177 (1995).
D. Ishii, T. Takeuchi, J. Chromatogr. Sci.22, 400 (1984).
P. R. Dluzneski, J. W. Jorgenson, J. High Resolut. Chromatogr. Chromatogr. Commun.8, 248 (1985).
S. Folestad, B. Josefsson, M. Larsson, J. Chromatogr.391, 347 (1987).
R. Swart, J. C. Kraak, H. Poppe, Chromatographia40, 587 (1995).
K. Gohlin, M. Larsson, J. Chromatogr.634, 31 (1993).
K. Gohlin, M. Larsson, J. Chromatogr.645, 41 (1993).
R. Swart, J. C. Kraak, H. Poppe, J. Chromatogr. A670, 25 (1994).
R. Swart, J. C. Kraak, H. Poppe, Chromatographia40, 587 (1996).
P. P. H. Tock, G. Stegeman, R. Peerboom, H. Poppe, J. C. Kraak, K. K. Unger, Chromatographia24, 617 (1987).
P. P. H. Tock, C. Boshoven, H. Poppe, J. C. Kraak, K. K. Unger, J. Chromatogr.477, 95 (1989).
A. L. Crego, J. C. Diez-Masa, M. V. Dabrio, Anal. Chem.65, 1615 (1993).
Y. Guo, L. A. Colón, Anal. Chem.67, 2511 (1995).
Y. Guo, L. A. Colón, J. Microcol. Sep.7, 485 (1995).
L. L. Hench, J. K. West Chem. Rev.90, 33 (1990).
C. J. Brinker, G. W. Scherer, “Sol-Gel Science”, Academic Press, New York, 1989.
M. S. Bello, R. Rezzonico, P. G. Righetti, Science266, 773 (1994).
K. K. Unger (Ed), “Packings and Stationary Phases in Chromatographic Techniques”, Marcel Dekker, New York, 1990.
C. F. Poole, S. K. Poole, “Chromatography Today”, Elsevier, New York, 1993.
“Standard Reference Material Certificate”, National Institute of Science and Technology, USA, 1990.
S. A. Wise, L. C. Sanders, J. High Resolut. Chromatogr. Chromatogr. Commun.8, 248 (1985).
N. Tanaka, H. Goodell, B. L. Karger, J. Chromatogr.158, 233 (1978).
K. B. Sentell, J. G. Dorsey, J. Chromatogr.461, 193 (1989).
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Guo, Y., Colón, L.A. Open tubular liquid chromatography using a sol-gel derived stationary phase. Chromatographia 43, 477–483 (1996). https://doi.org/10.1007/BF02292997
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DOI: https://doi.org/10.1007/BF02292997