Summary
A sensitive capillary gas chromatographic method is described for the simultaneous determination of lidocaine, tetracaine, procaine and dibucaine. The method was applied to the determination of anesthetics in tissue homogenates incubated at 38°C at doses between 10 and 400 mg/kg. In the liver tissue thein vitro metabolization of the studied anesthetics is most rapid for tetracaine, also fast for procaine, while for lidocaine and dibucaine the metabolization is very slow. In brain tissue thein vitro metabolization of anesthetics is very slow.
The method shows good analytical parameters: linearity between 5 and 40 μg/ml; day-to-day reproducibility ca. 8% for a concentration of 20 μg/ml, precision ca. 7% for a concentration of 20μg/ml. Accuracy is also very good.
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Culea, M., Palibroda, N., Moldovan, Z. et al. Gas chromatographic study of some local anesthetics. Chromatographia 28, 24–26 (1989). https://doi.org/10.1007/BF02290377
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DOI: https://doi.org/10.1007/BF02290377