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Application of β-cyclodextrin for the analysis of estrogenic steroids in human urine by high-performance liquid chromatography

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Summary

A procedure is described for simultaneous determination of estriol, estrone and 17β-estradiol in human urine. After acid hydrolysis of the sulphate conjugates, the estrogens were extracted into diethyl ether. The ether extracts were concentrated and applied directly to an HPLC column. Using a 25 cm C-18 column and acetonitrile-water modified by the addition of β-cyclodextrin as mobile phase, the separation of estriol, estradiol and estrone was achieved within 20 minutes. The extraction of estrogens from the biological matrix is excellent. Estrogens were detected using a UV-detector (280 nm) or a spectrofluorimetric detector (λexc=280nm, λem=312 nm). The latter detection system has been used for the determination of estrogens in the urine of non-pregnant women. The procedure is simple and can be used in clinical practice.

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Authors and Affiliations

  1. Medical Center of Postgraduate Education, Department of Biopharmaceutics, Debowa 3, 85626, Bydgoszcz, Poland

    H. Lamparczyk

  2. Faculty of Pharmacy, Medical Academy, Hallera 107, 80416, Gdańsk, Poland

    P. K. Zarzycki, J. Nowakowska & R. J. Ochocka

Authors
  1. H. Lamparczyk
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  2. P. K. Zarzycki
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  3. J. Nowakowska
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  4. R. J. Ochocka
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Lamparczyk, H., Zarzycki, P.K., Nowakowska, J. et al. Application of β-cyclodextrin for the analysis of estrogenic steroids in human urine by high-performance liquid chromatography. Chromatographia 38, 168–172 (1994). https://doi.org/10.1007/BF02290331

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  • DOI: https://doi.org/10.1007/BF02290331

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