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Generation of sequence-tagged sites from Xp22.3 by isolating commonAlu-PCR products of radiation hybrids retaining overlapping human X chromosome fragments

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Abstract

Several human diseases have been mapped to Xp22.3 on the distal short arm of the human X chromosome, and many genes in this area have been found to be expressed from the inactive X chromosome. To facilitate physical mapping and characterization of this interesting region, we have constructed a battery of radiation hybrids containing human X chromosomal fragments, and isolated two hybrid clones with overlapping fragments of Xp22.3.Alu-PCR on these hybrids and identification of sequences common to both hybrids allowed the isolation of six sequence-tagged sites (STSs) from Xp22.3. Five of the STSs were mapped to individual YACs comprising a recently constructed contig of this region. These novel STSs are useful markers for further physical characterization of this part of the genome.

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Authors and Affiliations

  1. Department of Pediatrics, University of California, 94143, San Francisco, CA, USA

    I. A. Glass, E. C. Salido & L. J. Shapiro

  2. Division of Medical Genetics, Harbor-UCLA Medical Center, 90509, Torrance, CA, USA

    M. Passage, L. Bernatowicz, T. Mohandas & P. H. Yen

Authors
  1. I. A. Glass
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  2. M. Passage
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  3. L. Bernatowicz
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  4. E. C. Salido
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  5. T. Mohandas
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  6. P. H. Yen
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  7. L. J. Shapiro
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Glass, I.A., Passage, M., Bernatowicz, L. et al. Generation of sequence-tagged sites from Xp22.3 by isolating commonAlu-PCR products of radiation hybrids retaining overlapping human X chromosome fragments. Hum Genet 97, 604–610 (1996). https://doi.org/10.1007/BF02281869

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  • DOI: https://doi.org/10.1007/BF02281869

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