Summary
A simple and quantitative method for the preparation and high performance liquid chromatography (HPLC) of N-acylated fluoroquinolones has been developed. The acylation procedure was performed with four different acid anhydrides and found to be applicable to several fluoroquinolones. The acylated derivatives were chromatographed directly on a Nucleosil C18 column without the necessity of further sample manipulation. Detector response for the N-acetyl derivatives of norfloxacin, ciprofloxacin, sarafloxacin, and temafloxacin was linear to at least 50 μg/mL. The retention behavior of the N-acyl derivatives can be predicted by the length of the carbon chain of the amide.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Z. S. Budvari-Baronyl, G. Y. Szasz, K. Takacs-Novak, I. Hermecz, A. Lore, J. Liq. Chroamtogr.,14, 3411 (1991).
J. F. Bauer, L. Elrod, Jr., J. R. Formnarino, D. E. Heathcote, S. K. Krogh, C. L. Linton, B. J. Norris, J. E. Quick, Pharm. Res.,7, 1177 (1990) and references therein.
J. F. Bauer, S. Howard, A. Schmidt, J. Chromatogr.,514, 348 (1990).
D. J. Griggs, R. Wise, J. Antimicrob. Chemother.,24, 437 (1989).
W. Schoenfeld, J. Knoeller, K. D. Bremm, A. Dalhoff, B. Weber, W. Koenig, Mikrobiol. Hyg., Ser. A,261, 338 (1986).
A. J. N. Groeneveld, J. R. B. J. Brouwers, Pharm. Weekbl., Sci. Ed.,8, 79 (1986).
L. Elrod, Jr., C. L. Linton, B. P. Shelat, C. F. Wong, J. Chromatogr.,519, 125 (1990).
K. J. Wang, S. M. Chen, S. J. Lin, H. L. Wu, J. Chromatogr.,360, 443 (1986).
J. L. Wa, T. Nakagawa, T. Uno, J. Chromatogr.,157, 297 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morley, J.A., Elrod, L. Determination of fluoroquinolone antibacterials as N-acyl derivatives. Chromatographia 37, 295–299 (1993). https://doi.org/10.1007/BF02278637
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02278637