Abstract
Adenylyl cyclase activity plays a central role in the regulation of most cellular processes. At least eight different adenylyl cyclases have been identified, which are endowed with various and sometimes opposing regulatory properties. Recently we have localized the human genes encoding two of these adenylyl cyclases: the gene for type 11 adenylyl cyclase is located on chromosome 2 (sub-band 2p15.3), the gene for type VIII is located on chromosome 8 (sub-band 8824.2). More recently the type I gene has been located on chromosome 7 (sub-band 7pl2–7p13). Using in situ hybridization, we have now localized the genes for three other adenylyl cyclases: the type III gene has been localized on chromosome 2 in the sub-band 2p22–2p24, the type V gene on chromosome 3 at position 3q13.2–3q21, and the type VI gene on chromosome 12 at position 12q12–12q13. It therefore appears that all adenylyl cyclase genes, known at present are located on different chromosomes and thus are likely to be independently regulated.
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Abbreviations
- AC :
-
adenylyl cyclase
References
Bakalyar HA, Reed RR (1990) Identification of a specialized adenylyl cyclase that may mediate odorant detection. Science 250:1403–1406
Berry-Kravis E, Huttenlocher PR (1992) Cyclic AMP metabolism in fragile X syndrome. Ann Neurol 31:22–26
Blin N, Stafford DW (1976) A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res 9:2303–2308
Choi EJ, Xia Z, Storm DR (1992) Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin. Biochemistry 31:6492–6498
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
Feinstein PG, Schrader KA, Bakalyar HA, Tang WJ, Krupinski J, Gilman AG, Reed RR (1991) Molecular cloning and characterization of a Ca2+/calmoduline-insensitive adenylyl cyclase from rat brain. Proc Natl Acad Sci USA 88:10173–10177
Gao B, Gilman AG (1991) Cloning and expression of a widely distributed (type IV) adenylyl cyclase. Proc Natl Acad Sci USA 88:10178–10182
Giuili G, Roechel N, Scholl U, Mattei MG, Guellaen G (1993) Colocalization of the genes coding for the α3 and β3 subunits of soluble guanylyl cyclase to human chromosome 4 at 831.3-q33. Hum Genet 91:257–260
Greenberg SM, Castellucci VF, Bayley H, Schwartz JH (1987) A molecular mechanism for long term sensitization in Aplysia. Neuron 329:62–65
Ishikawa Y, Katsushika S, Chen L, Halnon NJ, Kawabe J, Homcy CJ (1992) Isolation and characterization of a novel cardiac adenylyl cyclase. J Biol Chem 267:13553–13557
Iyengar R (1993) Molecular and functional diversity of mammalian Gs-stimulated adenylyl cyclases. FASEB J 7:768–775
Katsushika S, Chen L, Kawabe JL, Nilakantan R, Halnon NJ, Homcy CJ, Ishikawa Y (1992) Cloning and characterization of a sixth adenylyl cyclase isoform: type V and type VI constitute a subgroup within the mammalian adenylyl cyclase family. Proc Natl Acad Sci USA 89:8774–8878
Krupinski J, Lehman PC, Frankenfield CD, Zwaagstra JC, Watson PA (1992) Molecular diversity in adenylyl cyclase family; evidence for eight forms of the enzyme and cloning of type 6. J Biol Chem 267:24858–24862
Levitzki A (1987) Regulation of hormone sensitive adenylyl cyclase. Trends Pharmacol Sci 8:299–303
Maniatis T, Fritsch EF, Sambrook J (eds) (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 388–389
Mattei MG, Philip N, Passage E, Moisan JP, Mandel JL, Mattei JF (1985) DNA probe localisation at 18 p113 band by in situ hybridization and identification of a small supernumerary chromosome. Hum Genet 69:268–271
Parma J, Stengel D, Gannage MJ, Poyard M, Barouki R, Hanoune J (1991) Sequence of a human brain adenylyl cyclase partial cDNA: Evidence for a consensus cyclase domain. Biochem Biophys Res Commun 179:455–462
Premont RT, Chen J, Ma HW, Ponnapalli M, Iyengar R (1992) Two members of a widely expressed subfamily of hormone stimulated adenylyl cyclases. Proc Natl Acad Sci USA 89:9808–9813
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Stengel D, Parma J, Gannage MH, Roekel N, Mattei MG, Barouki R, Hanoune J (1992) Different chromosomal localization of two adenylyl cyclase genes expressed in human brain. Hum Genet 90:126–130
Villacres EC, Xia Z, Bookbinder LH, Edelhoff S, Disteche CM, Storm DR (1993) Cloning, chromosomal mapping, and expression of human fetal brain type I adenylyl cyclase. Genomics 16:473–478
Wahl CR, Stern M, Stark G (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687
Xia Z, Choi EJ, Wang F, Storm DR (1992) The type III calcium/calmodulin sensitive adenylyl cyclase is not specific to olfactory sensory neurons. Neurosci Lett 144:169–173
Xia Z, Choi EJ, Wang F, Blazinski C, Storm DR (1993) The type I calmodulin sensitive adenylyl cyclase is neural specific. J Neurochem 60:305–311
Yoshimura M, Cooper DMF (1992) Cloning and expression of a Ca2+ inhibitable adenylyl cyclase from NCB-20 cells. Proc Natl Acad Sci USA 89:6716–6720
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Haber, N., Stengel, D., Defer, N. et al. Chromosomal mapping of human adenylyl cyclase genes type III, type V and type VI. Hum Genet 94, 69–73 (1994). https://doi.org/10.1007/BF02272844
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DOI: https://doi.org/10.1007/BF02272844