Abstract
Substances with antiaphid activity were extracted from the leaf surface of aphid-resistantN. benthamiana and purified. Sugar esters and diacyl glycerols were isolated from the chloroform extract, but only diacyl glycerols showed significant activity. However, the major activity was found in the water extract rather than in the chloroform extract. From the water extract calcium chloride was isolated as the most abundant active substance. Only calcium chloride showed significant activity among several calcium salts and chlorides of several metals that are abundant in plants. Calcium contents per unit area of leaf surface ofN. benthamiana and aphid-resistantN. gossei were almost equal to each other and 10–100 times higher than that of aphid-susceptibleN. tabacum.
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References
Corcuera, L. J. 1993. Biochemical basis for the resistance of barley to aphids.Phytochemistry 33:741–747.
Goffreda, J. C., Ave, D. A., Mutschler, M. A., Steffens, J. C., andTingey, W. M. 1989. Aphid deterrence by glucose esters in glandular trichomes of the wild tomato,Lycopersicon pennellii.J. Chem. Ecol. 15:2135–2147.
Hagimori, M., Matsui, M., Matsuzaki, T., Shinozaki, Y., Shinoda, Y., andHarada, H. 1993. Production of somatic hybrids betweenNicotiana benthamiana andN. tabacum and their resistance to aphids.Plant Sci. 91:213–222.
Huesing, J., Jones, D., DeVerna, J., Myers, J., Collins, G., Severson, R., andSisson, V. 1989. Biochemical investigations of antibiosis material in leaf exudate of wildNicotiana species and intraspecific hybrids.J. Chem. Ecol. 15:1203–1217.
Joshi, B. G., andSitaramaiah, S. 1975. Note on relative toxicity of someNicotiana species to tobacco caterpillarSpodoptera litura (Fabricius).Tob. Res. 1:86–87.
Matsuzaki, T., Shinozaki, Y., Hagimori, M., Tobita, T., Shigematsu, H., andKoiwai, A. 1992. Novel glycerolipids and glyco lipids from the surface lipids ofNicotiana benthamiana.Biosci. Biotech. Biochem. 56:1565–1569.
Ma, W. C. 1972.Meded. Landbouwhogesch. Wageningen 72(12):1–162.
Mittler, T. E., andKoski, P. 1974. Meridic artificial diets for rearing aphids.Entomol. Exp. Appl. 17:524–525.
Neal, J. J., Tingey, W. M., andSteffens, J. C. 1990. Sucrose esters of carboxylic acids in glandular trichomes ofSolanum berthaultii deter settling and probing by green peach aphid.J. Chem. Ecol. 16:487–497.
Pickett, J. A., Wadhams, L. J., andWoodcock, C. M. 1992. The chemical ecology of aphids.Annu. Rev. Entomol. 37:67–90.
Salas, M., Corcuera, L. J., andArgandona, V. H. 1990. Effect of potassium nitrate on gramine content and resistance of barley against the aphidSchizaphis graminum.Phytochemistry 29:3789–3791.
Severson, R. F., Chortyk, O. T., Stephenson, M. G., Pittarelli, G. W., Neal, J. W., Jr., Buta, J. G., Jackson, D. M., andSisson, V. A. 1992. Natural pesticides from the cuticular extract ofNicotiana gossei.Tob. Chem. Res. Conf. 46:39–40.
Thurston, R. 1961. Resistance inNicotiana to the green peach aphid and some other tobacco insect pests.J. Econ. Entomol. 54:946–949.
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Harada, H., Takahashi, H., Matsuzaki, T. et al. Calcium chloride as a major component contributing to aphid resistance ofNicotiana benthamiana . J Chem Ecol 22, 1579–1589 (1996). https://doi.org/10.1007/BF02272399
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DOI: https://doi.org/10.1007/BF02272399