Summary
A sensitive and stereoselective liquid chromatographic (HPLC) assay for the S and R enantiomers of furprofen in human plasma has been developed. The assay is based on derivatization with S(−)-1-phenylethylamine with formation of two diastereoisomeric derivatives and on their separation and quantitation using HPLC with uv detection. The method is linear from 25 to 600 ng ml−1 of both enantiomers, with a variation coefficient below 10.8%, and a detection limit of 20 ng ml−1. This procedure is suitable for pharmacokinetic studies.
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Carlucci, G., Mazzeo, P. & Palumbo, G. Indirect stereoselective determination of enantiomers of furprofen in human plasma by high-performance liquid chromatography. Chromatographia 34, 618–620 (1992). https://doi.org/10.1007/BF02269873
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DOI: https://doi.org/10.1007/BF02269873