Summary
The interfacing of a high performance liquid chromatograph, utilizing a dry-packed Spherisorb® C8 column (0.25×500 mm), with a four-sector tandem mass spectrometer via a frit-FAB probe is described. In order to utilize the sensitivity of the mass spectrometer, which is equipped with an array detector, the gradient solvent was split to a flow rate of 2–5 μL/min before the sample injector. The effluent flows directly into the mass spectrometer for the recording of the (M+H)+ ions of mixtures with the first of the two mass spectrometers (MS-1), or for their collision-induced-decomposition (CID) spectra by scanning the second mass spectrometer (MS-2). Those components that elute too close in time to allow the complete CID spectrum of each are diverted into a sample loop, which later can be switched in-line to the mass spectrometer to provide more time for collecting all the data. The method is demonstrated with a tryptic digest of the α-chain of human hemoglobin.
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Cappiello, A., Palma, P., Papayannopoulos, I.A. et al. Efficient introduction of HPLC fractions into a high performance tandem mass spectrometer. Chromatographia 30, 477–483 (1990). https://doi.org/10.1007/BF02269791
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DOI: https://doi.org/10.1007/BF02269791