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Determination of glycuronic acids by high-performance anion chromatography with pulsed amperometric detection

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Acid hydrolysis (0.25M H2SO4) coupled with enzyme catalysis (pectolyase and β-D-glucuronidase) were employed to extract galacturonic and glucuronic acids from microbial polysaccharides, plant residues, animal wastes, sewage sludge and soil. The glycuronic acids were separated by high-performance anion chromatography (HPAC) on a strong anion-exchange column using 0.1M sodium hydroxide with 0.25M sodium acetate as the mobile phase and determined by pulsed amperometric detection (PAD). HPAC-PAD was found to be superior to high-performance liquid chromatography with ultra-violet (UV) detection in terms of resolution and sensitivity of glycuronic acids. HPAC-PAD was not subject to interferences present with low UV detection (210 nm) and was highly selective for glycuronic acids. Enzymatic hydrolysis after treatment with mild acid (0.25M H2SO4) released galacturonic acids from organge peel and pectin, while glucuronic acid was released from Acacia powder. Large amounts of glycuronic acids were also extracted from plant materials. Low levels of uronic acids were detected in poultry manure, sewage sludge and organic-amended soils.

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Authors and Affiliations

  1. Department of Soil and Environmental Sciences, University of California, 92521, Riverside, CA, USA

    D. A. Martens & W. T. Frankenberger Jr.

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  1. D. A. Martens
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  2. W. T. Frankenberger Jr.
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Corrected version of paper published in Vol. 30, No. 5/6, September 1990, pp. 249–254

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Martens, D.A., Frankenberger, W.T. Determination of glycuronic acids by high-performance anion chromatography with pulsed amperometric detection. Chromatographia 30, 651–656 (1990). https://doi.org/10.1007/BF02269740

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  • DOI: https://doi.org/10.1007/BF02269740

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