Skip to main content
SpringerLink
Log in

High-performance anion-exchange chromatography of carbohydrates using a new resin and pulsed amperometric detection

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

This paper reports the results of a study on the use of a new polymer-based, strong anion-exchange, stationary phase for rapid and selective separation of carbohydrates and related compounds by high-pH, anion-exchange chromatography with pulsed amperometric detection. The new adsorbent has been obtained by direct nitration of 2.8 μm, spherical non-porous highly crosslinked, styrene-divinylbenzene copolymer beads, followed by reduction of superficially introduced nitro groups with nascent hydrogen and quaternization of the resultant amino groups with iodomethane. It is reported that by optimizing the ionic strength of the mobile phase, columns packed with the new anion-exchanger can be successfully employed to separate, either in isocratic or gradient elution mode, oligosaccharides, positional isomers of gluco-disaccharides, as well as uronic acids and sugar monophosphates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. R. Hardy, R. R. Townsend, Proc. Natl. Acad. Sci. USA85, 3289 (1988).

    Google Scholar 

  2. Y. L. Lee, Anal. Biochem.189, 151 (1990).

    Google Scholar 

  3. D. C. Johnson, D. Dobberpuhl, R. Roberts, P. Vandeberg, J. Chromatogr.640, 79 (1993).

    Google Scholar 

  4. R. D. Rocklin, C. A. Pohl, J. Liq. Chromatogr.6, 1577 (1983).

    Google Scholar 

  5. D. Olechno, S. R. Carter, W. T. Edwards, D. G. Gillen, Am. Biotechnol. Lab.5, 38 (1987).

    Google Scholar 

  6. J. R. Stillian, C. A. Pohl, J. Chromatogr.499, 249 (1990).

    Google Scholar 

  7. R. E. Majors, LC-GC Int.6, 193 (1993).

    Google Scholar 

  8. D. P. Lee, J. Chromatogr. Sci.22, 327 (1984).

    Google Scholar 

  9. D. P. Lee, M. T. Bunker, J. Chromatogr. Sci.27, 496 (1989).

    Google Scholar 

  10. K. Mopper, C. A. Shultz, L. Chevolot, C. Germain, R. Revuelta, R. Dowson, Envirom. Sci. Technol.26, 133 (1992).

    Google Scholar 

  11. C. Corradini, D. Corradini, C. G. Huber, G. K. Bonn, J. Chromatogr.685, 213 (1994).

    Google Scholar 

  12. C. G. Huber, P. J. Oefner, G. K. Bonn Anal. Biochem.212, 351 (1993).

    Google Scholar 

  13. S. V. Prabhu, R. P. Baldwin, J. Chromatogr.503, 227 (1990).

    Google Scholar 

  14. H.-P. Lieker, K. Thielecke, K. Buchlholz, P. J. Reilly Carbohydr. Res.238, 307 (1993).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Cromatografia del C.N.R., Area della Ricerca di Roma, P.O. Box 10, 00016, Monterotondo Stazione (Rome), Italy

    C. Corradini & D. Corradini

  2. Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, 6020, Innsbruck, Austria

    C. G. Huber & G. K. Bonn

Authors
  1. C. Corradini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Corradini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. G. Huber
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. K. Bonn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Corradini, C., Corradini, D., Huber, C.G. et al. High-performance anion-exchange chromatography of carbohydrates using a new resin and pulsed amperometric detection. Chromatographia 41, 511–515 (1995). https://doi.org/10.1007/BF02269712

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02269712

Key Words

Search

Navigation