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Gradient thin-layer chromatography of coumarins and furocoumarins

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Summary

Extracts of four umbelliferous plants containing coumarins and furocoumarins were chromatographed in sandwich chambers with a glass distributor. best separations were obtained for stepwise gradient elution in the concentration range of 2.5 to 15% ethyl acetate in chloroform. Seven eluent fractions with increasing ethyl acetate concentrations were introduced directly under the distributor of the sandwich chamber.

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Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Medical Academy, Staszica 6, 20-081, Lublin, Poland

    G. Matysik, M. Bieganowska & E. Soczewiński

  2. Department of Pharmacognosy, Medical Academy, Staszica 6, 20-081, Lublin, Poland

    K. Glowniak

Authors
  1. K. Glowniak
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  2. G. Matysik
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  3. M. Bieganowska
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  4. E. Soczewiński
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Glowniak, K., Matysik, G., Bieganowska, M. et al. Gradient thin-layer chromatography of coumarins and furocoumarins. Chromatographia 22, 307–310 (1986). https://doi.org/10.1007/BF02268780

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  • DOI: https://doi.org/10.1007/BF02268780

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