Skip to main content
SpringerLink
Log in

High-Performance Thin-Layer Chromatography Analysis for Simultaneous Quantifications of Phenolic Biomarkers in Oxalis corniculata L. Methanolic Fraction

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Summary

Oxalis corniculata is an important medicinal plant, but chromatographic analysis for the simultaneous quantification of different phenolic compounds has not been performed till date in this plant species. A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination of caffeic acid, vanillic acid, and syringic acid in O. corniculata L. methanolic fraction was developed for the first time. In HPTLC, for achieving good separation, the mobile phase of toluene—ethyl acetate—formic acid (7:3:1, v/v) was used. The densitometric determination was carried out at 300 nm in reflection/absorption mode. The calibration curves were linear in the range of 100–700 ng per spot for caffeic acid, vanillic acid, and syringic acid. HPTLC analysis has indicated the presence of optimum amount of caffeic acid (0.025 ± 0.0002), vanillic acid (0.018 ± 0.0005), and syringic acid (0.04 ± 0.0006) in the methanolic fractions. The optimized method was successfully applied for the analysis of three major phenolics in O. corniculata L. The proposed method is simple, precise, specific, and accurate. The quantification of these phenolic compounds has not yet been reported in this species which may be utilized for the proper standardization of the drug.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K.R. Kirtikar, B.D. Basu, Indian Medicinal Plants, Vol. 1, 3rd edn., MS Periodical Experts, New Delhi, 1975, p. 437.

    Google Scholar 

  2. K.S. Madhava, K. Sivaji, R.K. Tulasi, Flowering Plants of Chitoor District, Andhra Pradesh, India, Student Offset Printers, Tirupati, 2008, p. 54.

    Google Scholar 

  3. I.S. Mohammad, A.K. Mir, J. Ethnopharmacol. 69 (2000) 48–56.

    Google Scholar 

  4. A. Kathiriya, K. Das, E.P. Kumar, K.B. Mathai, Iranian J. Cancer Prevention 4 (2010) 157–165.

    Google Scholar 

  5. M. Hioki, T. Kaori, Yokotani, Y. Kunijiro, J. Plant Res. 121 (2008) 133–136.

    Article  Google Scholar 

  6. C.P. Khare, Indian Medicinal Plants: An Illustrated Dictionary, Springer Verlag, Berlin/Heidelberg, 2007, p. 457.

    Book  Google Scholar 

  7. M.P. Raghvendra, S. Satish, K.A. Raveesha, My Science 1 (2006) 72–78.

    Google Scholar 

  8. B.G. Unni, A. Borah, Asian J. Exp. Sci. 23 (2009) 103–108.

    CAS  Google Scholar 

  9. S.J. Patil, S.B. Patil, Bioscan 1 (2006) 63–67.

    Google Scholar 

  10. D. Manna, P.K. Dutta, B. Achari, A.A. Lohia, Antimicrob. Agents Chemother. 54 (2010) 4825–4832.

    Article  CAS  Google Scholar 

  11. S. Li, C. Long, F. Liu, S. Lee, Q. Guo, R. Li, Y. Liu, J. Ethnopharmacol. 108 (2006) 59–67.

    Article  Google Scholar 

  12. A. Gurib-Fakim, M. Sewraj, J. Gueho, E. Dulloo, J. Ethnopharmacol. 39 (1993) 175–185.

    Article  CAS  Google Scholar 

  13. S.S. Hebbar, V.H. Harsha, V. Shripati, G.R. Hedge, J. Ethnopharmacol. 94 (2004) 261–266.

    Article  CAS  Google Scholar 

  14. M.C. Divakar, S. Lakshmidevi, N. Sreenivasan, Indian Drugs 49 (2012) 30–39.

    Google Scholar 

  15. S. Sagi, B. Avula, Y.H. Wang, J. Zhao, I.A. Khan, J. Sep. Sci. 37 (2014) 2797–2804.

    Article  CAS  Google Scholar 

  16. S. Verma, A. Gupta, M.V. Ramana, A.K.S. Rawat, J. Planar Chromatogr. 29 (2016) 356–360.

    Article  CAS  Google Scholar 

  17. A. Gupta, S. Verma, S. Khatoon, H. Dwivedi, A.K.S. Rawat, J. Planar Chromatogr. 28 (2015) 452–457.

    Article  CAS  Google Scholar 

  18. C. Calixto-Campos, T.T. Carvalho, M.S.N. Hohmann, F.A. Pinho-Ribeiro, V. Fattori, M.F. Manchope, A.C. Zarpelon, M.M. Baracat, S.R. Georgetti, R. Casagrande, W.A. Verri Jr., J. Nat. Prod. 78 (2015) 1799–1808.

    Article  CAS  Google Scholar 

  19. C. Magnani, V.L.B. Isaac, M.A. Correa, H.R.N. Salgado, Anal. Methods 6 (2014) 3203.

    Article  CAS  Google Scholar 

  20. X. Wei, D. Chen, Y. Yi, H. Qi, X. Gao, H. Fang, Q. Gu, L. Wang, L. Gu, J. Evid. Based Complementary Altern. Med. 2012 (2012) 426537.

    Google Scholar 

  21. ICH-Q2A, Text on Validation of Analytical Procedures, Harmonised Tripartite Guideline prepared within the International Conference on Harmonisation of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Geneva, 1994.

  22. ICH-Q2B, Validation of Analytical Procedures: Methadology, Harmonised Tripartite Guideline Prepared within the International Conference on Harmonisation of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Geneva, 1996.

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacy, Pranveer Singh institute of Technology, Kanpur, India

    Abhishek Gupta, Prashant Kumar, Omprakash Goshain & Awani Kumar Rai

  2. Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, India

    Jyotsana Dwivedi

Authors
  1. Abhishek Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Prashant Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Omprakash Goshain
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Awani Kumar Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jyotsana Dwivedi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Abhishek Gupta.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gupta, A., Kumar, P., Goshain, O. et al. High-Performance Thin-Layer Chromatography Analysis for Simultaneous Quantifications of Phenolic Biomarkers in Oxalis corniculata L. Methanolic Fraction. JPC-J Planar Chromat 31, 327–331 (2018). https://doi.org/10.1556/1006.2018.31.4.8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1556/1006.2018.31.4.8

Key Words

Search

Navigation