Summary
A new HPLC stationary phase was synthesized by thein situ covalent immobilization of human serum albumin (HSA). The protein was immobilized on a commerically available diol column which had been activated with 1,1-carbonyldiimidazole. Initial chromatographic studies show that this phase can be used for chiral separations of enantiomeric solutes and that these separations may reflectin vitro binding to the HSA. The effects of mobile phase composition and temperature on the stereochemical resolutions are reported.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
W. E. Muller, in “Drug Stereochemistry: Analytical Methods and Pharmacology”,I. W. Wainer andD. E. Drayer, eds., Marcel Dekker, 1988; pp. 227–244.
S. Allenmark, B. Bomgren, H. Boren, J. Chromatogr.,264, 63 (1983).
J. Hermansson, J. Chromatogr.,269, 71 (1983).
I. W. Wainer, in “Drug Stereochemistry: Analytical Methods and Pharmacology”,I. W. Wainer andD. E. Drayer, eds. Marcel Dekker, 1988; pp. 147–173.
A. C. Mehta, J. Chromatogr.,426, 1 (1988).
Y.-Q. Chu, I. W. Wainer, Pharm. Res.,5, 680 (1988).
I. W. Wainer, R. M. Stiffin, J. Chromatogr.,424, 158 (1988).
Y.-Q. Chu, I. W. Wainer, J. Chromatogr.,497, 191 (1989).
R. C. Jewell, K. L. R. Brouwer, P. J. McNamara, J. Chromatogr.,487, 257 (1989).
N. Lammers, H. de Bree, C. P. Groen, H. M. Ruijten, B. J. de Jong, in “Bioanalysis of Drugs and Metabolites, Especially Anti-inflammatory and Cardiovascular”,E. Reiud, J. D. Robinson andI. D. Wilson, eds., Plenum Press, 1988; pp. 303–303.
I. W. Wainer, in “Chiral separations, Volume II”, D. Stevenson and I. D. Wilson, eds., Plenum Press, in press.
G. Félix, M. Liu, Bio-Sciences,8, 2 (1989).
S. C. Crowley, R. R. Walters, J. Chromatogr.,226, 157 (1983).
C. Bertucci, E. Domenici, G. Uccello-Barretta, P. Salvadori, J. Chromatogr., submitted.
S. C. Bell, R. J. McCaully, C. Gochman, S. J. Childress, M. I. Gluckman, J. Med. Chem.,11, 457 (1968).
A. Corbella, P. Gariboldi, G. Jommi, A. Forgione, F. Marcucci, P. Martelli, E. Mussini, E. Mauri, J. Chem. Soc. Chem. Commun., 721 (1973).
C. Bertucci, C. Rosini, D. Pini, P. Salvadori, J. Pharm. Biomed. Anal.,5, 171 (1987).
R. A. Thompson, S. Andersson, S. Allenmark, J. Chromatogr.,465, 263 (1989).
S. Allenmark, J. Liq. Chromatogr.,9, 425 (1986).
S. Allenmark, S. Andersson, Chirality,1, 154 (1989).
A. Allenmark, B. Bomgren, H. Boren, J. Chromatogr.,264, 63 (1983).
C. Lagercrantz, T. Larsson, I. Denfors, Comp. Biochem. Physiol (C),69, 375 (1981).
A. Jaulmes, C. Vidal-Madjar, in “Advances in Chromatography”,J. C. Giddings, E. Grushka, P. R. Brown, eds., Marcel Dekker, 1989, pp. 1–64.
J. J. Kirkland, W. W. Yau, H. J. Stoklosa, C. H. Dilks, Jr., J. Chromatogr. Sci.,15, 303 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Domenici, E., Bertucci, C., Salvadori, P. et al. Synthesis and chromatographic properties of an HPLC chiral stationary phase based upon human serum albumin. Chromatographia 29, 170–176 (1990). https://doi.org/10.1007/BF02268706
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02268706