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A normal phase HPTLC method for the quantitative determination of fumonisin B1 in rice

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Summary

A normal phase HPTLC method has been validated by spiking, in quadruplet, uncontaminated extract of rice with fumonisin B1 over the range 0 to 16μg/g. The method utilises solid phase extraction using strong anionic exchange (SAX) cartridges, uni-directional normal phase high performance thin layer chromatography (HPTLC), novel visualisation by dipping into a 0.16% acidic solution ofp-anisaldehyde and quantification by scanning fluoro-densitometry. Response was linear only over the range 0 to 5 μg/g (0 to 125 ng/spot) where recoveries averaged 81% for rice. Weighted linear regression yielded a limit of detection of 0.25 μg/g for rice. Coefficients of variance were 15.4, 5.3, 2.8, 3.5, and 0.9% at fumonisin B1 levels of 0.20, 0.5, 1.0, 2.0 and 5 μg/g respectively, demonstrating good precision. This method claims to be the first fully quantitative HPTLC method for determining fumonisin B1 in rice.

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Authors and Affiliations

  1. Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, New Elephant Road, 1205, Dhaka, Bangladesh

    M. Dawlatana

  2. Chatham Maritime, Natural Resources Institute, Central Avenue, ME4 4TB, Chatham, Kent, UK

    R. D. Coker & M. J. Nagler

  3. School of Pharmacy and Biomedical Sciences, University of Portsmouth, King Henry I Street, PO1 2DZ, Portsmouth, UK

    G. Blunden

Authors
  1. M. Dawlatana
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  2. R. D. Coker
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  3. M. J. Nagler
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  4. G. Blunden
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Dawlatana, M., Coker, R.D., Nagler, M.J. et al. A normal phase HPTLC method for the quantitative determination of fumonisin B1 in rice. Chromatographia 41, 187–190 (1995). https://doi.org/10.1007/BF02267953

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  • DOI: https://doi.org/10.1007/BF02267953

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