Abstract
A high performance liquid chromatography (HPLC) based micro-method for estimation of indole-3-acetic acid (IAA) levels in single half nodes from the flowering stalks ofAvena fatua has been developed; this features a dual electrode coulometric electrochemical detector operating at a detection limit of c. 2 pg. Samples were prepared by solvent partitioning and preliminary fractionation with C18 Sep-Pak cartridges. Two stages of reversed phase ion pair HPLC were employed; the first was gradient elution with fluorescent detection, the second, isocratic elution with coulometric detection. The lower limit for estimation of IAA levels in purified extracts was c. 5 pg.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akiyama M, Sakurai N, Kuraishi S (1983) A simplified method for quantitative determination of indoleacetic acid by high performance liquid chromatography with a fluorometric detector. Plant Cell Physiol 24:1431–1439
Blakesley D, Allsopp AJA, Hall JF, Weston GD, Elliot MC (1984) Use of reversed phase ion-pair high-performance liquid chromatography for the removal of compounds inhibitory to the formation of the 2-methyl indolo-α-pyrone derivative of indole-3-acetic acid. J Chromatogr 294:480–484
Crozier A, Loferski K, Zaerr JB, Morris RO (1980) Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures. Planta 150:366–370
Dutrieu J, Delmotte YA (1983) Dual electrochemical detection in HPLC, new trend in analytical chemistry. Analytical Chem 314:416–417
Frei RW, Michel L, Santi W (1976) Post-column fluorescence derivatisation of peptides: problems and potential in high-performance liquid chromatography. J Chromatogr 126:665–677
Jensen E, Junttila O (1982) Indolyl-3-acetic acid from shoots ofSalix pentandra. Physiologia Plantarum 56:241–244
Law DM, Hamilton RH (1982) A rapid isotope dilution method for analysis of indole-3-acetic acid and indoleacetyl aspartic acid from small amounts of plant tissue. Biochem Biophys Res Comm 106:1035–1041
McClintock SA, Purdy WC (1984) Liquid chromatography/electrochemical detection: a review. International Laboratory 14:70–76
Mousdale DMA (1981) Reversed-phase ion-pair high-performance liquid chromatography of the plant hormones indolyl-3-acetic acid and abscisic acid. J Chromatogr 209:489–493
Roe DK (1983) Comparison of amperometric and coulometric electrochemical detectors for HPLC through a figure of merit. Analytical letters 16:613–631
Sandberg G, Dunberg A (1982) Precision and accuracy of indole-3-acetic acid analyses performed with the 2-methyl indolo-α-pyrone fluorescence assay and with a high performance liquid chromatography technique with spectrofluorimetric detection exemplified on pine tissue (Pinus sylvestris L.). Physiologia Plantarum 55:315–322
Sweetser PB, Swartzfager DG (1978) Indole-3-acetic acid levels of plant tissue as determined by a new high performance liquid chromatographic method. Plant Physiol 61:254–258
Wright M (1981) Reversal of the polarity of IAA transport in the leaf sheath base ofEchinochloa colonum. J Exp Bot 32:159–169
Wright M, Osborne DJ (1977) Gravity regulation of cell elongation in nodes of the grassEchinochloa colonum. Biochem Physiol Pflanzen 171:479–492
Wright M, Mousdale DMA, Osborne DJ (1978) Evidence for a gravity-regulated level of endogenous auxin controlling cell elongation and ethylene production during geotropic bending in grass nodes. Biochem Physiol Pflanzen 172:581–596
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wright, M., Doherty, P. Auxin levels in single half nodes ofAvena fatua estimated using high performance liquid chromatography with coulometric detection. J Plant Growth Regul 4, 91–100 (1985). https://doi.org/10.1007/BF02266947
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02266947