Abstract
The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callus of Douglas-fir. The data are also used to illustrate a procedure whereby the accuracy of chromatographic analyses can be verified within definable probability limits.
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Abbreviations
- GC-MS:
-
combined gas chromatography-mass spectrometry
- HPLC:
-
high performance liquid chromatography
- IAA:
-
indole-3-acetic acid
- SEC:
-
steric exclusion chromatography
- SICM:
-
selected ion current monitoring
References
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Technical Paper No. 5379 from the Oregon State University Agricultural Experiment Station
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Crozier, A., Loferski, K., Zaerr, J.B. et al. Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures. Planta 150, 366–370 (1980). https://doi.org/10.1007/BF00390171
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DOI: https://doi.org/10.1007/BF00390171