Skip to main content
SpringerLink
Log in

Modulation of arginine decarboxylase activity in cucumber (Cucumis sativus) cotyledons in short-term organ culture

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Among the various amines administered to excisedCucumis sativus cotyledons in short-term organ culture, agmatine (AGM) inhibited arginine decarboxylase (ADC) activity to around 50%, and putrescine was the most potent entity in this regard. Homoarginine (HARG) dramatically stimulated (3- to 4-fold) the enzyme activity. Both AGM inhibition and HARG stimulation of ADC were transient, the maximum response being elicited at 12 h of culture. Mixing experiments ruled out involvement of a macromolecular effector in the observed modulation of ADC. HARG-stimulated ADC activity was completely abolished by cycloheximide, whereas AGM-mediated inhibition was unaffected. Half-life of the enzyme did not alter on treatment with either HARG or AGM. The observed alterations in ADC activity are accompanied by change in Km of the enzyme. HARG-stimulated ADC activity is additive to that induced by benzyladenine (BA) whereas in presence of KCl, HARG failed to enhance ADC activity, thus demonstrating the overriding influence of K+ on amine metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altman A (1982) Retardation of radish leaf senescence by polyamines. Physiol Plant 54:189–193

    Article  CAS  Google Scholar 

  • Altman A, Kaur-Sawhney R, Galston AW (1977) Stabilization of oat leaf protoplasts through polyamine mediated inhibition of senescence. Plant Physiol 60:570–574

    Article  PubMed  CAS  Google Scholar 

  • Altman A, Friedman R, Amir D, Levin N. (1982) Polyamine effects and metabolism in plants under stress conditions. In: Wareing PF (ed) Plant growth subst proc int conf: Academic Press, New York, pp 483–494

    Google Scholar 

  • Altman A, Friedman R, Levin N (1983) Alternative metabolic pathways for polyamine biosynthesis in plant development. In: Bachrach U et al (eds) Advances in polyamine research, Vol. 4. Raven Press, New York, pp 395–408

    Google Scholar 

  • Bachrach U (1980) The induction of ornithine decarboxylase in normal and neoplastic cells. In: Gaugas JM (ed) Polyamines in biomedical research. Wiley, New York, pp 81–107

    Google Scholar 

  • Bagni N, Fracassini SD, Torrigiani P (1981) Polyamines and growth in higher plants. In: Caldarera CM et al (eds) Advances in polyamine research. Raven Press, New York, Vol. 3, pp 377–388

    Google Scholar 

  • Canellakis ES, Viceps-Madore D, Kyriakidis DA, Heller JS (1979) The regulation and function of ornithine decarboxylase and of polyamines. Curr Top Cell Regul 15:155–202

    PubMed  CAS  Google Scholar 

  • Chideckel EW, Edwards D (1983) Nonmetabolizable amino acids are potent stimulators of hepatic and renal ornithine decarboxylase activity. Biochem J 210:617–619

    PubMed  CAS  Google Scholar 

  • Clark JL, Fuller JL (1975) Regulation of ornithine decarboxylase in 3T3 cells by putrescine and spermidine: Indirect evidence for translational control. Biochemistry 14:4403–4409

    Article  PubMed  CAS  Google Scholar 

  • Feirer RP, Mignon G, Litvay JD (1984) Arginine decarboxylase and polyamines are required for embryogenesis in the wild carrot. Science 223:1433–1435

    Article  PubMed  CAS  Google Scholar 

  • Flores HE, Galston AW (1982) Polyamines and plant stress: Activation of putrescine biosynthesis by osmotic shock. Science 217:1259–1261

    Article  PubMed  CAS  Google Scholar 

  • Fong WF, Heller JS, Canellakis ES, (1976) The appearance of an ornithine decarboxylase inhibitory protein upon the addition of putrescine to cell cultures. Biochim Biophys Acta 428: 456–465

    PubMed  CAS  Google Scholar 

  • Galston AW (1983) Polyamines as modulators of plant development. Bioscience 33:382–388

    Article  CAS  Google Scholar 

  • Harik SI, Hollenberg MD, Snyder SH (1974) Ornithine decarboxylase turnover slowed by α-hydrazinoornithine. Mol Pharmacol 10:41–47

    PubMed  CAS  Google Scholar 

  • Heller JS, Fong WF, Canellakis ES (1976) Induction of a protein inhibitor to ornithine decarboxylase by the end products of its reaction. Proc Natl Acad Sci USA 73:1858–1862

    Article  PubMed  CAS  Google Scholar 

  • Kay JE, Lindsay VJ (1973) Control of ornithine decarboxylase activity in stimulated human lymphocytes by putrescine and spermidine. Biochem J 132:791–796.

    PubMed  CAS  Google Scholar 

  • Kuehn GD, Atmar VJ (1982) Post-translational control of ornithine decarboxylase by polyamine dependent protein kinase. Fed Proc 41:3078–3083

    PubMed  CAS  Google Scholar 

  • Lignowski EM, Splittstoesser WE (1971) Changes in arginine levels and metabolism of urea and ornithine inCucurbita moschata seedlings. Physiol Plant 25:225–229

    Article  CAS  Google Scholar 

  • Lowry CH, Rosebrough HJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • McCann PP (1980) Regulation of ornithine decarboxylase in eukaryotes. In: Gaugas JM (ed) Polyamines in biomedical research. Wiley, New York, pp. 109–124

    Google Scholar 

  • McCann PP, Tardif C, Duchense MC, Mamont PS (1977a) Effect of α-methylornithine on ornithine decarboxylase activity of rat hepatoma cells in culture. Biochem. Biophys Res Commun 76:893–899

    Article  PubMed  CAS  Google Scholar 

  • McCann PP, Tardif C, Mamont PS (1977b) Regulation of ornithine decarboxylase by ODC-antizyme in HTC cells. Biochem Biophys Res Commun 75:948–954

    Article  PubMed  CAS  Google Scholar 

  • Mitchell JLA (1983) Ornithine decarboxylase and ornithine decarboxylase modifying protein ofPhysarum polycephalum. Methods Enzymol 94:140–146

    Article  PubMed  CAS  Google Scholar 

  • Murthy KS, Smith TA, Bould C (1971) The relationship between putrescine content and potassium status of black currant leaves. Ann Bot 35:687–695

    Google Scholar 

  • Pegg AE (1979) Investigation of the turnover of rat liver S-adenosyl-L-methionine decarboxylase using a specific antibody. J Biol Chem 254:3249–3253

    PubMed  CAS  Google Scholar 

  • Ramakrishna S, Adiga PR (1975) Arginine decarboxylase fromLathyrus sativus seedlings: Purification and properties. Eur J Biochem 59:377–386

    Article  PubMed  CAS  Google Scholar 

  • Russell DH (1980) Ornithine decarboxylase as a biological and pharmacological tool. Pharmacology 20:117–129

    Article  PubMed  Google Scholar 

  • Russell DH (1981) Post-translational modification of ornithine decarboxylase by its product. Biochem Biophys Res Commun 99:1167–1172

    Article  PubMed  CAS  Google Scholar 

  • Smith TA (1975) Recent advances in the biochemistry of plant amines. Phytochemistry 14:865–890

    Article  CAS  Google Scholar 

  • Smith TA, Sinclair C (1967) The effect of acid feeding on amine formation in barley. Ann Bot 31:103–111

    CAS  Google Scholar 

  • Srivenugopal KS, Adiga PR (1981) Enzymic conversion on agmatine to putrescine inLathyrus sativus seedlings: Purification and properties of a multifunctional enzyme (putrescine synthase). J Biol Chem 256:9532–9541

    PubMed  CAS  Google Scholar 

  • Srivenugopal KS, Adiga PR (1983) Preparation and purification of N-carbamyl putrescine and [ureido-14C]N-carbamyl putrescine. Methods Enzymol 93:429–430

    Article  Google Scholar 

  • Suresh MR, Ramakrishna S, Adiga PR (1978) Regulation of arginine decarboxylase and putrescine levels inCucumis sativus cotyledons. Phytochemistry 17:57–63

    Article  CAS  Google Scholar 

  • Viceps-Madore D, Chen KY, Tsou H, Canellakis ES (1982) Studies on the role of protein synthesis of sodium on the regulation of ornithine decarboxylase activity. Biochim Biophys Acta 717:305–315

    PubMed  CAS  Google Scholar 

  • Wright JM, Boyle SM (1982) Negative control of ornithine decarboxylase and arginine decarboxylase by cAMP inEscherichia coli. Mol Gen Genet 186:482–487

    Article  PubMed  CAS  Google Scholar 

  • Young ND, Galston AW (1983) Putrescine and acid stress. Plant Physiol 71:767–771

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India

    G. L. Prasad & P. R. Adiga

Authors
  1. G. L. Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. R. Adiga
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prasad, G.L., Adiga, P.R. Modulation of arginine decarboxylase activity in cucumber (Cucumis sativus) cotyledons in short-term organ culture. J Plant Growth Regul 4, 49–61 (1985). https://doi.org/10.1007/BF02266943

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02266943

Keywords

Search

Navigation