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Are proteasomes involved in the formation of the kinetochore?

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Abstract

Proteasomes catalyse the degradation of proteins responsible for the regulation of mitosis enabling the cell to complete cell division. We have studied the effect of an inhibitor of the chymotrypsin-like activity of the proteasome on the trilaminar structure of the kinetochore in HeLa cells. Whereas a role for the proteasome in the degeneration of the kinetochore was predicted, we found instead that the inhibitor strongly retarded kinetochore development. We observed different ‘developmental’ stages of the kinetochore from the fibrous ball of a ‘prekinetochore’ to the ‘mature’ kinetochore in one cell. The data presented here support the proposition that proteasomes are involved in kinetochore formation.

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References

  • Cooke CA, Heck MM, Earnshaw WC (1987) The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.J Cell Biol 105: 2053–2067.

    Article  PubMed  Google Scholar 

  • Downes CS, Mullinger AM, Johnson RT (1991) Inhibitors of DNA topoisomerase II prevent sister chromatid separation in mammalian cells but do not prevent exit from mitosis.Proc Natl Acad Sci USA 88: 8895–8899.

    PubMed  Google Scholar 

  • Earnshaw WC, Bernat RL (1990) Chromosomal passengers: towards an integrated view of mitosis.Chromosoma 100: 139–146.

    Article  Google Scholar 

  • Figuereido-Pereira ME, Berg KA, Wilk S (1994) A new inhibitor of the chymotrypsin-like activity in the multicatalytic proteinase complex (20S proteasome) induces accumulation of ubiquitin-protein conjugates in a neuronal cell.J Neurochem 63: 1578–1581.

    PubMed  Google Scholar 

  • Ghosh S, Paweletz N (1987) Kinetochores on chromosomes enclosed within the nuclear envelope.Cell Biol Int Rep 11: 165–169.

    Article  PubMed  Google Scholar 

  • Glotzer M, Murray AW, Kirschner MW (1991) Cyclin is degraded by the ubiquitin pathway.Nature 349: 132–138.

    Article  PubMed  Google Scholar 

  • Griscavage JM, Wilk S, Ignarro LJ (1995) Serine and cysteine proteinase inhibitors prevent nitric oxide production by activated macrophages by interfering with transcription of the inducible NO synthase gene.Biochem Biophys Res Commun 215: 721–729.

    Article  PubMed  Google Scholar 

  • Hayden JH, Bowser SS, Rieder CL (1990) Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt lung cells.J Cell Biol 111: 1039–1045.

    Article  PubMed  Google Scholar 

  • Heneen WK (1975) Ultrastructure of the prophase kinetochore in cultured cells of rat-kangaroo (Potorous tridactylis).Hereditas 79: 209–220.

    PubMed  Google Scholar 

  • Hepler PK (1980) Membranes in the mitotic apparatus of barley cells.J Cell Biol 86: 490–499.

    Article  PubMed  Google Scholar 

  • Holloway SL (1995) Sister chromatid separation in vivo and in vitro.Curr Opin Genet Dev 5: 243–248.

    Article  PubMed  Google Scholar 

  • Holloway SL, Glotzer M, King RW, Murray AW (1993) Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.Cell 73: 1393–1402.

    Article  PubMed  Google Scholar 

  • Kaufman SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG (1993) Specific proteolytic cleavage of poly (ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis.Cancer Res 53: 3976–3985.

    PubMed  Google Scholar 

  • Lamprecht J, Schroeter D, Paweletz N (1990) Mitosis arrested by deuterium oxide. Light microscopic, immunofluorescence and ultrastructural characterization.Eur J Cell Biol 51: 303–312.

    PubMed  Google Scholar 

  • Merdes A, De Mey J (1990) The mechanism of kinetochorespindle attachment and polewards movement analysed in PtK2 cells at the prophase-prometaphase transition.Eur J Cell Biol 53: 313–325.

    PubMed  Google Scholar 

  • Pagano M, Tam SW, Theodoras AM et al. (1995) Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27.Science 269: 682–685.

    PubMed  Google Scholar 

  • Palombella VJ, Rando OJ, Goldberg AL, Maniatis T (1994). The ubiquitin-proteasome pathway is required for processing of the NF-kB1 precursor protein and the activation of NF-kB.Cell 78: 773–785.

    Article  PubMed  Google Scholar 

  • Paweletz N, Schroeter D, Finze E-M (1994) Making first contacts between the spindle and the chromosomes in HeLa cells.Chrom Res 2: 115–122.

    Article  PubMed  Google Scholar 

  • Pfarr CM, Cove M, Grissom PM et al. (1990) Cytoplasmic dynein is localized to kinetochores during mitosis.Nature 345: 263–265.

    Article  PubMed  Google Scholar 

  • Pines J, Hunter T (1991) Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.J Cell Biol 115: 1–17.

    Article  PubMed  Google Scholar 

  • Rieder CL (1982) The formation, structure and composition of the mammlian kinetochore and kinetochore fiber.Int Rev Cytol 79: 1–58.

    PubMed  Google Scholar 

  • Rieder CL, Alexander SP, Rupp G (1990) Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells.J Cell Biol 110: 81–95.

    Article  PubMed  Google Scholar 

  • Roos UP (1973) Light and electron microscopy of rat kangaroo cells in mitosis. II. Kinetochore structure and function.Chromosoma 41: 195–220

    Article  PubMed  Google Scholar 

  • Saitoh H, Tomkiel J, Cooke CA et al. (1992) CENP-C, an autoantigen in Scleroderma, is a component of the human inner kinetochore plate.Cell 70: 115–125.

    Article  PubMed  Google Scholar 

  • Schroeter D, Paweletz N, Finze E-M, Kiesewetter U-L (1993). Development of kinetochores during early mitosis in HeLa cells and the stability of a trilaminar kinetochore structure. In: Vig, BK, ed.Chromosome Segregation and Aneuploidy, NATO SI-Series H Vol 72. Heidelberg: Springer, pp 241–255.

    Google Scholar 

  • Spurr AR (1969) A low-viscosity epoxy-resin embedding medium for electron microscopy.J Ultrastruct Res 26: 31–43.

    Article  PubMed  Google Scholar 

  • Steuer ER, Wordeman L, Schroer TA, Sheetz MP (1990) Localization of cytoplasmic dynein to mitotic spindles and kinetochores.Nature 345: 266–268.

    Article  PubMed  Google Scholar 

  • Tomkiel J, Cooke CA, Saitoh H, Bernat RL, Earnshaw WC (1994) CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase.J Cell Biol 125: 531–545.

    Article  PubMed  Google Scholar 

  • Vig BK, Richards B, Paweletz N (1993) The mammalian centromere: centromere separation, kinetochore proteins and aneupolody. In: Obe G, ed.Advances in Mutagenesis Research Vol. 4. Heidelberg: Springer, pp. 169–203.

    Google Scholar 

  • Wilk S, Figuereido-Pereira M (1993) Synthetic inhibitors of the multicatalytic proteinase complex (proteasome).Enz Prot 47: 306–313.

    Google Scholar 

  • Wójcik C, Schroeter D, Wilk S, Lamprecht, J, Paweletz N (1996a) Ubiquitin-mediated proteolysis centers in HeLa cells: indication from studies of an inhibitor of the chymotrypsin-like activity of the proteasome.Eur J Cell Biol (in press).

  • Wójcik C, Schroeter D, Stoehr M, Wilk S, Paweletz N (1996b) An inhibitor of the chymotrypsin-like activity of the multicatalytic proteinase complex (20S proteasome) induces arrest in G2 and metaphase in HeLa cells.Eur J Cell Biol 70: 172–178.

    PubMed  Google Scholar 

  • Wordeman L, Mitchison TJ (1995) Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis.J Cell Biol 128: 95–105.

    Article  PubMed  Google Scholar 

  • Yen TJ, Li G, Schaar BT, Szilak I, Cleveland DN (1992) CENPE is a putative kinetochore motor that accumulates just before mitosis.Nature 359: 536–539.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division 0430, German Cancer Research Center, Im Neuenheimer Feld 280, 69120, Heidelberg

    N. Paweletz, D. Schroeter & E. -M. Finze

  2. Department of Histology and Embryology, Institute of Biostructure, Warsaw Medical Academy, Warsaw, Poland

    C. Wójcik

Authors
  1. N. Paweletz
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  2. C. Wójcik
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  3. D. Schroeter
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  4. E. -M. Finze
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Dedicated to the memory of Prof. Dr. Daniel Mazia, a fatherly friend.

accepted for publication by H. C. Macgregor

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Paweletz, N., Wójcik, C., Schroeter, D. et al. Are proteasomes involved in the formation of the kinetochore?. Chromosome Res 4, 436–442 (1996). https://doi.org/10.1007/BF02265050

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  • DOI: https://doi.org/10.1007/BF02265050

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