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Estimation of energy metabolism in human skeletal muscle homogenate as a diagnostic aid

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Journal of Inherited Metabolic Disease

Abstract

A method is presented for thein vitro testing of the energy metabolism of human skeletal muscle. The levels of creatine phosphate, ATP, ADP and AMP, which are defined by the activity of many enzyme systems, are measured after incubation of the muscle homogenate with various substrates in the presence or absence of specific inhibitors. Detection of abnormal metabolite concentrations is important for diagnosis of patients with myopathic syndromes.

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References

  • Bergmeyer, H. U.Methoden der Enzymatischen Analyse, Verlag Chemie, Weinheim, 1974

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  • Bookelman, H., Trijbels, J. M. F., Sengers, R. C. A., Janssen, A. J. M., Veerkamp, J. H. and Stadhouders, A. M. Pyruvate oxidation in rat and human skeletal muscle mitochondria.Biochem. Med. 20 (1978) 395–403

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Authors and Affiliations

  1. Departments of Pediatrics and Submicroscopic Morphology, University of Nijmegen, PO Box 9101, 6500 HB, Nijmegen, The Netherlands

    W. Ruitenbeek, R. C. A. Sengers, J. M. F. Trijbels, A. M. Stadhouders & A. J. M. Janssen

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  1. W. Ruitenbeek
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  2. R. C. A. Sengers
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  3. J. M. F. Trijbels
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  4. A. M. Stadhouders
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  5. A. J. M. Janssen
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Ruitenbeek, W., Sengers, R.C.A., Trijbels, J.M.F. et al. Estimation of energy metabolism in human skeletal muscle homogenate as a diagnostic aid. J Inherit Metab Dis 4, 91–92 (1981). https://doi.org/10.1007/BF02263606

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  • DOI: https://doi.org/10.1007/BF02263606

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