Skip to main content
SpringerLink
Log in

Standardization of test conditions for high performance liquid chromatography columns

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Comparisons of columns, column packings and column packing methods are made difficult and sometimes invalidated by differences and inadequacies in the test procedures used and the experimental data recorded. This paper reviews test procedures and recommends standards for a) the experimental and test parameters which must be recorded in order to enable comparisons to be made from laboratory to laboratory, b) the group of chromatographic parameters which best represent column performance for comparative purposes, with methods for their calculation, c) test solutes and eluents for some different types of packing materials. A computer program in BASIC is given which converts the experimental parameters into relevant chromatographic parameters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. Golay, “Gas Chromatography 1958”, Ed.Desty, Butterworths, London 1959, p. 36.

    Google Scholar 

  2. J. C. Giddings. J. Chromatog.13, 301 (1964).

    Article  Google Scholar 

  3. J. C. Giddings, “Dynamics of Chromatography Pt. 1”, Marcel Dekker, New York 1965.

    Google Scholar 

  4. J. H. Knox andM. Saleem, J. Chromatog. Sci.7, 614 (1969).

    Google Scholar 

  5. J. N. Done, G. J. Kennedy andJ. H. Knox, Gas Chromatography 1972”, Institute of Petroleum, London 1972, p. 145.

    Google Scholar 

  6. J. H. Knox, Ann. Rev. Phys. Chem.24, 29 (1973).

    Article  Google Scholar 

  7. E. Grushka, L. R. Snyder andJ. H. Knox, J. Chromatog. Sci.13, 25 (1975).

    Google Scholar 

  8. R. G. Laird, J. Jurand andJ. H. Knox, Proc. Soc. Analyt. Chem. 310 (1974).

  9. L. C. Hansen andR. E. Sievers, J. Chromatog.99, 123 (1974).

    Article  Google Scholar 

  10. J. H. Knox andG. Vasvari, J. Chromatog.83, 181 (1973).

    Article  Google Scholar 

  11. D. S. Horne, J. H. Knox andL. McLaren, Sepn. Sci.1, 531 (1966).

    Google Scholar 

  12. J. H. Stefano andH. C. Beachell, J. Chromatog. Sci.10, 481, 654 (1972).

    Google Scholar 

  13. J. H. Knox, G. R. Laird andP. A. Raven, J, Chromatog.122, 129 (1976).

    Google Scholar 

  14. C. R. Wike andP. Chang, Amer. Inst. Chem. Engr. J.1, 264 (1955).

    Google Scholar 

  15. W. Heyduk andS. C. Cheng, Chem. Engng. Sci.26, 635 (1971).

    Article  Google Scholar 

  16. T. H. Sherwood, R. L. Pigford andC. R. Wilke, “Mass Transfer”, McGraw-Hill Book Co. New York 1975, p. 25.

    Google Scholar 

  17. Internatinal Critical Tables.

Download references

Author information

Authors and Affiliations

  1. Pharmaceuticals Division, Hurdsfield Industrial Estate, SK 10 2NA, Macclesfield, Cheshire, England

    Paul A. Bristow ICI

  2. Wolfson Liquid Chromatography Unit, Department of Chemistry, University of Edinburgh, West Mains Road, EH9 3JJ, Edinburgh, Scottland

    John H. Knox

Authors
  1. Paul A. Bristow ICI
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John H. Knox
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bristow, P.A., Knox, J.H. Standardization of test conditions for high performance liquid chromatography columns. Chromatographia 10, 279–289 (1977). https://doi.org/10.1007/BF02263001

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02263001

Keywords

Search

Navigation