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Photochemical reaction detection of phylloquinone and menaquinones. A comparison with chemical post-columns reduction for fluorescence detection

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Summary

A new photochemical reaction method for the on-line fluorimetric detection of natural vitamin K homologs was developed. In addition to its high sensitivity, the method features an interesting selectivity for the determination of these compounds in a complex matrix such as human serum. The formation of different photolysis products as a function of reaction conditions, and the optimalisation of a segmented flow reaction system will be discussed. The results indicate that the method is 4 times more sensitive than UV detection, and at least as selective as fluorescence detection after post-column chemical reduction.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, State University of Ghent, A.Z., De Pintelaan, 135, B-9000, Ghent, Belgium

    M. F. Lefevere

  2. Department of Analytical Chemistry, Free University, De Boelelaan 1083, NL-1081 HV, Amsterdam, the Netherlands

    R. W. Frei, A. H. M. T. Scholten & U. A. Th. Brinkman

Authors
  1. M. F. Lefevere
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  2. R. W. Frei
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  3. A. H. M. T. Scholten
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  4. U. A. Th. Brinkman
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Lefevere, M.F., Frei, R.W., Scholten, A.H.M.T. et al. Photochemical reaction detection of phylloquinone and menaquinones. A comparison with chemical post-columns reduction for fluorescence detection. Chromatographia 15, 459–467 (1982). https://doi.org/10.1007/BF02261609

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