Summary
Experience in blood and tissue drug assays by means of HPLC with UV-VIS detection is reported. More than 20 drugs or their analogues, which belong to many different therapeutic classes, are analyzed with a limited number of columns and of eluents (isocratic mode). A simple device to warm a reverse-phase column has also been tested. The guidelines applied here will be of interest to people who want to simplify the analysis of a wide range of drugs.
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Internal files from Laboratoires Fabre (Castres, France).
C. Legendre, personal communication Laboratoires Fournier (Dijon, France).
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Desager, J.P., Sclavons, M. HPLC with UV-VIS spectrophotometric detection for blood and tissue drug assays. Chromatographia 15, 451–452 (1982). https://doi.org/10.1007/BF02261607
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DOI: https://doi.org/10.1007/BF02261607