Summary
In many analytical separation problems the retention time is not sufficient to identify the compounds of interest. This is especially true with complex mixtures where additional analytical data are necessary to confirm positively the identity of the separated components. An analytical high-pressure liquid chromatograph has been coupled to a UV/Visible spectrophotometer and a mass spectrometer and used for the separation and subsequent identification of compounds in different plant extracts. The results show that the direct combination of HPLC with on-line detection techniques can be successfully used in the analysis of complex mixtures giving positive identification of components and an overall reduction in analysis time.
Similar content being viewed by others
References
Attillio Melera, Technical Paper No. MS-10, Hewiett-Packard.
R. Schuster, Anal. Chem.52, No. 4 (1980).
T. J. Mabry, K. R. Markham, M. B. Thomas, The Systematic Identification of Flavonoids, Springer Verlag.
G. Guiochon, p. J. Arpino, Anal. Chem.51, No. 7 (1979).
A. M. Verney, A. M. Debelmas, Annales Pharmaceutiques, Francaises6, 475 (1973).
K. Hostettmann, ETH Zürich, private communication 1980.
P. Hupe, H. Lauer, K. Zech, Chromatographia13, 413 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schuster, R. Separation and positive identification of compounds in complex sample mixtures using on-line, LC-UV/VIS and LC-MS, direct coupling techniques. Chromatographia 13, 379–385 (1980). https://doi.org/10.1007/BF02261517
Issue Date:
DOI: https://doi.org/10.1007/BF02261517