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HPLC determination of catabolic amines in silage using their dansyl derivatives and an electrochemical detector

  • Late Papers from the 17th International Symposium on Chromatography, Vienna, Austria, September 25–30, 1988
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Summary

The use of the electrochemical detector (EC) in the high performance liquid chromatography (HPLC) of dansyl derivatives of biogenic amines is reported. Isocratic and gradient elution patterns of synthetic mixtures of putrescine, cadaverine, 1,6-diaminohexane, tryptamine, histamine, tyramine, spermine and spermidine on a reversed phase column are shown. Hydrodynamic voltammograms of standard compounds are presented. The detection limits of the EC are compared with those of ultraviolet and spectrofluorimetric detectors. A chromatographic profile of amines from the proteolytic catabolism of maize silage byClostridia is shown.

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Authors and Affiliations

  1. Dipartimento di Chimica “G. Ciamician”, Università di Bologna, Via Selmi, 2, 40126, Bologna, Italy

    G. Chiavari

  2. Centro di Studio per la Conservazione dei Foraggi, Consiglio Nazionale delle Ricerche, Via F.Re, 8, 40126, Bologna, Italy

    G. C. Galletti

  3. Presidio Multizonale Prevenzione, U.S.L. 28, Via Triachini, 17, 40138, Bologna, Italy

    Patrizia Vitali

Authors
  1. G. Chiavari
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  2. G. C. Galletti
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  3. Patrizia Vitali
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Chiavari, G., Galletti, G.C. & Vitali, P. HPLC determination of catabolic amines in silage using their dansyl derivatives and an electrochemical detector. Chromatographia 27, 216–220 (1989). https://doi.org/10.1007/BF02260449

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  • DOI: https://doi.org/10.1007/BF02260449

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